日本牛群中伪牛痘病毒的首次分离及基因特征分析
First isolation and genetic characterization of pseudocowpox virus from cattle in Japan.
作者信息
Ohtani Akifumi, Yokoyama Akihiro, Narushige Hisato, Inoshima Yasuo
机构信息
Yamaguchi Chubu Livestock Hygiene Service Center, 671-5 Kagawa, Yamaguchi, Yamaguchi, 754-0897, Japan.
Laboratory of Food and Environmental Hygiene, Cooperative Department of Veterinary Medicine, Gifu University, 1-1 Yanagido, Gifu, Gifu, 501-1193, Japan.
出版信息
Virol J. 2017 Sep 6;14(1):172. doi: 10.1186/s12985-017-0840-3.
BACKGROUND
Pseudocowpox virus (PCPV) infects cattle worldwide with zoonotic potential but has not been isolated in Japan. Thus, the epidemiological status of PCPV infection in cattle is undetermined.
RESULTS
In May 2016, a cattle in a farm in Yamaguchi Prefecture showed white vesicles and hyperemia in the mucosa under the tongue surface, but not on the teats and coronary cushions. A parapoxvirus was isolated from the oral lesion swab and was genetically characterized based on the full-length sequence of B2L gene encoding viral envelope. Phylogenetic analysis showed that the isolated virus was classified into PCPV.
CONCLUSION
This case indicates its potential spread in Japan. This is the first report of isolation of PCPV in Japan.
背景
伪牛痘病毒（PCPV）在全球范围内感染牛，具有人畜共患病传播潜力，但在日本尚未分离到该病毒。因此，牛群中PCPV感染的流行病学状况尚不确定。
结果
2016年5月，山口县一个农场的一头牛在舌面下方黏膜处出现白色水疱和充血，但乳头和冠状垫未出现。从口腔病变拭子中分离出一种副痘病毒，并根据编码病毒包膜的B2L基因的全长序列进行了基因特征分析。系统发育分析表明，分离出的病毒被归类为PCPV。
结论
该病例表明其在日本有潜在传播风险。这是日本首次分离出PCPV的报告。
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