Martin Tesmine, Lou Yuan-Chao, Aryal Sarita, Tai Jung-Hsiang, Chen Chinpan
Institute of Biomedical Sciences, Academia Sinica, Taipei, 115, Taiwan, ROC.
Chemical Biology and Molecular Biophysics, Taiwan International Graduate Program, Academia Sinica, Taipei, 115, Taiwan, ROC.
Biomol NMR Assign. 2018 Apr;12(1):27-30. doi: 10.1007/s12104-017-9774-3. Epub 2017 Sep 5.
Cyclophilins are peptidyl prolyl isomerases that play an important role in a wide variety of biological functions like protein folding and trafficking, intracellular and extracellular signaling pathways, nuclear translocation and in pre-mRNA splicing. Two cyclophilins have been identified in the parasitic organism Trichomonas vaginalis and were named as TvCyP1 and TvCyP2. The 2 enzymes have been found to interact with Myb transcription factors in the parasite which regulate the iron induced expression of ap65-1 gene leading to cytoadherence of the parasite to human vaginal epithelial cells to cause the disease trichomoniasis. TvCyP2 was found to interact specifically with Myb3 to regulate nuclear translocation of the transcription factor. It would be intriguing to identify the binding site of both proteins as it could pave way to newer targets for drug discovery. Here we report the H, C and N resonance assignments and secondary structure information of TvCyP2 that could help us investigate the interaction between Myb3 and TvCyP2 in detail using NMR.
亲环蛋白是肽基脯氨酰异构酶，在多种生物学功能中发挥重要作用，如蛋白质折叠与运输、细胞内和细胞外信号通路、核转运以及前体mRNA剪接。在寄生生物阴道毛滴虫中已鉴定出两种亲环蛋白，分别命名为TvCyP1和TvCyP2。已发现这两种酶与该寄生虫中的Myb转录因子相互作用，Myb转录因子调节铁诱导的ap65 - 1基因表达，导致寄生虫与人阴道上皮细胞的细胞粘附，从而引发滴虫病。发现TvCyP2与Myb3特异性相互作用以调节转录因子的核转运。确定这两种蛋白质的结合位点将很有趣，因为这可能为新药发现开辟新的靶点。在此，我们报告TvCyP2的氢、碳和氮共振归属以及二级结构信息，这有助于我们利用核磁共振详细研究Myb3与TvCyP2之间的相互作用。
