Zhang Shu-Li, Wang Yi-Ping, Du Zhen-Xia, Xue Meng, Zhang Jiao, Ma Qun
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China.
School of Science, Beijing University of Chemical Technology, Beijing 100102, China.
Zhongguo Zhong Yao Za Zhi. 2016 Mar;41(6):1135-1139. doi: 10.4268/cjcmm20160627.
To explore the differences in lipid metabolites in serum of hyperuricemic rats induced by fructose and normal rats by using lipid metabolomics technology, and screen the potential biomarkers related to hyperuricemia. The metabolic fingerprint spectrum of the serum in hyperuricemic rats(model group) and normal rats(control group) was obtained and analyzed by using ultra performance convergence chromatography-tandem-Q-time of flight mass spectrometry(UPC ² -Q/TOF-MS) method and the differences of metabolic spectra between two groups were compared via the multivariate statistical methods to screen differential metabolites. The results indicated that there was significant difference in metabolic spectra between model group and control group, and 11 differential metabolites were screened. Then eight potential biomarkers such as arachidonic acid, palmitic acid, oleic acid and linoleic acid were tentatively identified by using the exact mass number and secondary mass spectrometry(MS/MS spectrum). Therefore, a new research method for lipid metabolomics in serum of hyperuricemic rats induced by fructose was established successfully based on UPC ² -Q/TOF-MS. What's more, it was speculated that the abnormal metabolism of fatty acid might be associated with the pathogenesis of hyperuricemia, which would provide scientific basis for early detection and prevention of hyperuricemia.
采用脂质代谢组学技术探讨果糖诱导的高尿酸血症大鼠与正常大鼠血清脂质代谢产物的差异，筛选与高尿酸血症相关的潜在生物标志物。采用超高效合相色谱-串联-四极杆飞行时间质谱（UPC²-Q/TOF-MS）法获取并分析高尿酸血症大鼠（模型组）和正常大鼠（对照组）血清的代谢指纹图谱，通过多元统计方法比较两组代谢谱差异，筛选差异代谢产物。结果表明，模型组与对照组代谢谱存在显著差异，筛选出11种差异代谢产物。然后利用精确分子量和二级质谱（MS/MS谱）初步鉴定了花生四烯酸、棕榈酸、油酸和亚油酸等8种潜在生物标志物。因此，成功建立了基于UPC²-Q/TOF-MS的果糖诱导高尿酸血症大鼠血清脂质代谢组学研究新方法。此外，推测脂肪酸代谢异常可能与高尿酸血症的发病机制有关，为高尿酸血症的早期检测和预防提供科学依据。
