Faculty of Engineering, Hokkaido University , N13W8, Kita-ku, Sapporo 060-8628, Japan.
Graduate School of Chemical Sciences and Engineering, Hokkaido University , N13W8, Kita-ku, Sapporo 060-8628, Japan.
Biomacromolecules. 2017 Oct 9;18(10):3432-3438. doi: 10.1021/acs.biomac.7b01100. Epub 2017 Sep 19.
Nanofibrillated bacterial cellulose (NFBC) is produced by culturing a cellulose-producing bacterium (Gluconacetobacter intermedius NEDO-01) with rotation or agitation in medium supplemented with carboxymethylcellulose (CMC). Despite a high yield and dispersibility in water, the product immediately aggregates in organic solvents. To broaden its applicability, we prepared amphiphilic NFBC by culturing strain NEDO-01 in medium supplemented with hydroxyethylcellulose or hydroxypropylcellulose instead of CMC. Transmission electron microscopy analysis revealed that the resultant materials (HE-NFBC and HP-NFBC, respectively) comprised relatively uniform fibers with diameters of 33 ± 7 and 42 ± 8 nm, respectively. HP-NFBC was dispersible in polar organic solvents such as methanol, acetone, isopropyl alcohol, acetonitrile, tetrahydrofuran (THF), and dimethylformamide, and was also dispersible in poly(methyl methacrylate) (PMMA) by solvent mixing using THF. HP-NFBC/PMMA composite films were highly transparent and had a higher tensile strength than neat PMMA film. Thus, HP-NFBC has a broad range of applications, including as a filler material.
纳米原纤化细菌纤维素 (NFBC) 是通过在含有羧甲基纤维素 (CMC) 的培养基中旋转或搅拌培养产纤维素细菌 (中间醋酸杆菌 NEDO-01) 生产的。尽管产量高且在水中分散性好，但该产品在有机溶剂中会立即聚集。为了拓宽其应用范围，我们通过在含有羟乙基纤维素或羟丙基纤维素而不是 CMC 的培养基中培养菌株 NEDO-01 来制备两亲性 NFBC。透射电子显微镜分析表明，所得材料（分别为 HE-NFBC 和 HP-NFBC）分别由直径为 33 ± 7nm 和 42 ± 8nm 的相对均匀纤维组成。HP-NFBC 可分散在甲醇、丙酮、异丙醇、乙腈、四氢呋喃 (THF) 和二甲基甲酰胺等极性有机溶剂中，并可通过使用 THF 的溶剂混合分散在聚甲基丙烯酸甲酯 (PMMA) 中。HP-NFBC/PMMA 复合膜具有高透明度和高于纯 PMMA 膜的拉伸强度。因此，HP-NFBC 具有广泛的应用，包括作为填充材料。
