Micha Aikaterini-Eftychia, Psarras Kyriakos, Ouroumidis Odysseas, Siska Evangelia, Vlachaki Efthymia, Lymperopoulos Aristotelis, Symeonidis Nikolaos, Nikolaidou Christina, Venizelos Ioannis, Koliakos George, Pavlidis Theodoros E
1 Aristotle University School of Medicine, Thessaloniki, Greece.
2 General Hospital "Hippokrateion," Thessaloniki, Greece.
Surg Innov. 2017 Dec;24(6):543-551. doi: 10.1177/1553350617729510. Epub 2017 Sep 7.
To investigate the early and late antiadhesive effect and any changes of fibrin matrix regulation enzymes on rat peritoneum, after local administration of bevacizumab.
Rats were subjected to cecal abrasion. Bevacizumab (5 mg/kg) against placebo was given intraperitoneally. On the 2nd, 14th, and 28th postoperative days adhesions were scored, and tissue plasminogen activator (tPA), plasminogen activator inhibitor-1 (PAI-1), matrix metalloproteinase-9 (MMP-9), degree of fibrosis, and angiogenesis were measured in abrased cecum and in intact parietal peritoneum.
Bevacizumab significantly reduced adhesions up to 15% on the 2nd, 52.5% on the 14th, and 55% on the 28th postoperative day, and significantly increased tPA concentrations in peritoneum. PAI-1 was decreased, and a significantly higher tPA/PAI-1 ratio along with an increase of MMP-9 was measured at all time points. Fibrosis and angiogenesis were significantly lower on the 14th and 28th postoperative days.
Local bevacizumab administration has a strong early and late antiadhesive action on rat peritoneum, mediated by changes in the tPA/PAI-1 and MMP balance in favor of fibrinolysis up to 28 days after operations.
研究局部给予贝伐单抗后，其对大鼠腹膜的早期和晚期抗粘连作用以及纤维蛋白基质调节酶的任何变化。
对大鼠进行盲肠擦伤。将贝伐单抗（5毫克/千克）与安慰剂腹腔内给药。在术后第2天、第14天和第28天对粘连进行评分，并测量擦伤盲肠和完整壁层腹膜中的组织型纤溶酶原激活剂（tPA）、纤溶酶原激活剂抑制剂-1（PAI-1）、基质金属蛋白酶-9（MMP-9）、纤维化程度和血管生成情况。
贝伐单抗在术后第2天显著降低粘连达15%，第14天降低52.5%，第28天降低55%，并显著提高腹膜中tPA浓度。PAI-1降低，在所有时间点均测得显著更高的tPA/PAI-1比值以及MMP-9增加。术后第14天和第28天纤维化和血管生成显著降低。
局部给予贝伐单抗对大鼠腹膜具有强大的早期和晚期抗粘连作用，这是由tPA/PAI-1和MMP平衡的变化介导的，有利于术后28天内的纤维蛋白溶解。
