乌干达穆拉戈国家转诊医院分离出的肠杆菌科细菌中广谱β-内酰胺酶的表型流行情况
Phenotypic prevalence of extended spectrum beta-lactamases among enterobacteriaceae isolated at Mulago National Referral Hospital: Uganda.
作者信息
Ampaire Lucas, Nduhura Emmanuel, Wewedru Izale
机构信息
Department of Medical Laboratory Sciences, Mbarara University of Science and Technology, P.O Box. 1410, Mbarara, Uganda.
Microbiology Department, Mulago National Referral Hospital, Kampala, Uganda.
出版信息
BMC Res Notes. 2017 Sep 6;10(1):448. doi: 10.1186/s13104-017-2786-3.
Abstract
OBJECTIVE
Enterobacteriaceae, common causes of health care associated and community acquired infections are mainly treated with beta-lactam agents. Our study objective was to determine the prevalence and common enterobacteriaceae pathogen producing extended spectrum beta lactamases (ESBLs). The isolates were recovered from various clinical specimens. This was cross sectional study conducted between July 2016 and September 2016 at Mulago National Referral Hospital, Uganda. We used ChromID™ ESBL agar (Biomerieux SA, Lyon, France) and Vitek2 compact system GN83 card (BioMerieux Inc, Hazelwood, Missouri, USA) to detect and confirm presence of phenotypic extended spectrum beta lactamases producing pathogens respectively.
RESULTS
Of the 261 tested clinical isolates, 35 (13.4%) were identified as ESBLs producing bacteria. Escherichia coli predominated in the samples [18 (51.4%)], presenting the highest frequency of ESBLs producing, followed by Klebsiella pneumonia [10 (28.5%)], Proteus mirabilis [4 (11.4%)], Enterobacter sp. [2 (5.7%)] and least among Acinetobacter baumanii [1 (2.8%)].
摘要
目的
肠杆菌科细菌是医疗保健相关感染和社区获得性感染的常见病因，主要用β-内酰胺类药物治疗。我们的研究目的是确定产生超广谱β-内酰胺酶（ESBLs）的肠杆菌科病原菌的流行情况和常见类型。分离菌株取自各种临床标本。这是一项于2016年7月至2016年9月在乌干达穆拉戈国家转诊医院进行的横断面研究。我们分别使用ChromID™ ESBL琼脂（法国生物梅里埃公司，里昂）和Vitek2 compact系统GN83卡（美国密苏里州黑兹尔伍德市生物梅里埃公司）来检测和确认产生表型超广谱β-内酰胺酶的病原菌的存在。
结果
在261份测试的临床分离菌株中，35株（13.4%）被鉴定为产ESBLs细菌。样本中大肠杆菌占主导地位[18株（51.4%）]，产ESBLs频率最高；其次是肺炎克雷伯菌[10株（28.5%）]、奇异变形杆菌[4株（11.4%）]、肠杆菌属[2株（5.7%）]，鲍曼不动杆菌最少[1株（2.8%）]。
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