Department of Otolaryngology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Republic of Korea.
Sci Rep. 2017 Sep 6;7(1):10667. doi: 10.1038/s41598-017-10991-x.
Cell sheets with pre-vascularization have recently been developed but remain relatively untested in oral wound healing. Therefore, we examined the potential utility of our newly developed pre-vascularized mucosal cell sheets in oral wound healing. Mucosal keratinocytes, fibroblasts, and endothelial progenitor cells were primarily cultured for in vitro cell expansion from mucosa and blood of Sprague-Dawley rats. Mucosal cell sheets were generated using cultured keratinocytes and plasma fibrin (K sheet) or keratinocytes and a mixture of fibrin, fibroblasts, and endothelial cells (PV sheet). Autologous sheets were transplanted on deep wounds in the buccal region of rats. The gross and histological characteristics of wound healing were compared among control wound, K sheet, and PV sheet groups. We successfully cultured and expanded keratinocytes, fibroblasts, and endothelial progenitor cells in vitro for generating mucosal cell sheets with or without pre-vascularization. In the in vivo oral wound model, compared with the control wound, the PV sheet group exhibited rapid wound closure more prominently than the K sheet group. The histological healing in the PV sheet group was similar to that in rat normal buccal mucosa without fibrosis. The pre-vascularized mucosal cell sheet exhibited in vivo efficacy in oral wound healing by promoting accelerated healing.
细胞片具有预血管化功能，最近已经被开发出来，但在口腔伤口愈合方面的应用仍相对未经测试。因此，我们研究了我们新开发的预血管化黏膜细胞片在口腔伤口愈合中的潜在应用。黏膜角质形成细胞、成纤维细胞和内皮祖细胞主要从 Sprague-Dawley 大鼠的黏膜和血液中进行体外细胞扩增培养。使用培养的角质形成细胞和等离子纤维蛋白（K 片）或角质形成细胞和纤维蛋白、成纤维细胞和内皮细胞混合物（PV 片）生成黏膜细胞片。将自体片移植到大鼠颊部的深部伤口上。比较对照组伤口、K 片和 PV 片组的伤口愈合的大体和组织学特征。我们成功地在体外培养和扩增了角质形成细胞、成纤维细胞和内皮祖细胞，以生成具有或不具有预血管化功能的黏膜细胞片。在体内口腔伤口模型中，与对照组伤口相比，PV 片组的伤口闭合速度明显快于 K 片组。在 PV 片组中，组织学愈合类似于大鼠正常颊黏膜，没有纤维化。预血管化的黏膜细胞片通过促进愈合加速在口腔伤口愈合中表现出体内疗效。
