Akami Mazarin, Chakira Hamada, Andongma Awawing A, Khaeso Kanjana, Gbaye Olajire A, Nicolas Njintang Y, Nukenine E-N, Niu Chang-Ying
Department of Biological Sciences, Faculty of Science, University of Ngaoundere, PO Box 454, Ngaoundere, Cameroon.
College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
R Soc Open Sci. 2017 Aug 23;4(8):170692. doi: 10.1098/rsos.170692. eCollection 2017 Aug.
The intensive use of synthetic pesticides in cowpea storage has led to the development of resistance by and subsequent degradation of grain quality. In an attempt to circumvent these constraints, the susceptibility of to 2,2-dichlorovinyldimethyl phosphate (DDVP) and essential oil (EO) was investigated and variations in the proportions of nutritional values of treated grains 150 days after storage were assessed. The survival rate was recorded after five generations. The resistance index and biochemical parameters of grains were determined. The results from this study revealed that the survival rate and resistance index significantly increased proportionally with damage in DDVP treatments ( = 0.889; = 0.018) while in EO treatments, those values remained low without significant variations ( = 0.0764) throughout the generations. DDVP stored grains yielded higher crude protein values, but lower carbohydrates, tannins, phenolics and minerals compared to EO. Eighteen amino acids were detected in EO treated grains and 14 in DDVP which was devoid of albumin and prolamin. EO could therefore represent a safe alternative bio-pesticide to cope with insect resistance and enhance the nutritional qualities of stored cowpea seeds.
在豇豆储存中大量使用合成农药导致害虫产生抗药性,并进而使谷物品质下降。为了克服这些限制,研究了豇豆象对二氯二苯醚菊酯(DDVP)和精油(EO)的敏感性,并评估了储存150天后处理过的谷物营养价值比例的变化。记录了五代后的存活率。测定了谷物的抗性指数和生化参数。这项研究的结果表明,在DDVP处理中,存活率和抗性指数随损伤程度显著成比例增加(r = 0.889;P = 0.018),而在EO处理中,这些值在各代中一直较低,没有显著变化(r = 0.0764)。与EO相比,DDVP储存的谷物粗蛋白值较高,但碳水化合物、单宁、酚类和矿物质含量较低。在EO处理的谷物中检测到18种氨基酸,在DDVP处理的谷物中检测到14种,DDVP处理的谷物中不含白蛋白和醇溶蛋白。因此,EO可以作为一种安全的替代生物农药,以应对昆虫抗性并提高储存豇豆种子的营养品质。