Wang Qian, Yang Mei, Pei Jin, Wang Li, Wu Yi-Yun, Lv Hui
State Key Laboratory Breeding Base of System Research, Development and Utilization of Traditional Chinese Medicine Resources, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China.
Zhongguo Zhong Yao Za Zhi. 2016 Apr;41(7):1222-1226. doi: 10.4268/cjcmm20160711.
Effects of nine different moisture contents on vigor of Cyathula officinalis seeds and its anti-aging mechanism were studied by artificial accelerated aging through high temperature and wet. The research results showedthat seed vigor were generally decreased after artificial aging; in general, seed vigor and its anti-aging ability are relatively stronger within the scope of 6.55%-4.78% moisture content, the increase range of seed conductivity, peroxidase activity, malondialdehyde content,and reduce amplitude of activityof dehydrogenase , superoxide dismutaseare alllower as well. And when the moisture content reduced to 5.77%, all of the germination tests index of the non-aged seeds are the highest, and the activity of peroxidase the lowest,conductivity of leaching solution relatively low, activity of dehydrogenase and superoxide dismutase the highest,and catalase activityrelatively high.Therefore, in the low temperature germplasm preservation of C. officinalis seeds, the seed moisture content should be controlled close to the range of (5.70±1)% to keep higher vigor and anti-aging ability.
通过高温高湿人工加速老化的方法,研究了9种不同含水量对川牛膝种子活力及其抗衰老机制的影响。研究结果表明,人工老化后种子活力普遍下降;总体而言,在含水量6.55%-4.78%范围内,种子活力及其抗衰老能力相对较强,种子电导率、过氧化物酶活性、丙二醛含量的增加幅度以及脱氢酶、超氧化物歧化酶活性的降低幅度也均较低。当含水量降至5.77%时,未老化种子的各项发芽试验指标最高,过氧化物酶活性最低,浸出液电导率较低,脱氢酶和超氧化物歧化酶活性最高,过氧化氢酶活性相对较高。因此,在川牛膝种子低温种质保存中,种子含水量应控制在接近(5.70±1)%的范围内,以保持较高的活力和抗衰老能力。