Zhang Chun-Ping, He Ping, Du Dan-Dan, Wei Pin-Xiang, Yu Ze-Li, Xie Ying-Zan, Liu Hai-Ying
School of Life Sciences, Southwest University, Key Laboratory (Ministry of Education) of Eco-environments of Three Gorges Reservoir Region, Chongqing Key Laboratory of Plant Ecology and Resources Research for Three Gorges Reservoir Region, Chongqing 400715, China.
Zhong Yao Cai. 2011 May;34(5):665-9.
To explore the method of improving the salt resistance ability of seeds and seedlings of Perilla frutescens under NaCl stress.
Physiological indexes of Perilla frutescens seeds treated by sodium nitroprusside (SNP) under NaCl stress like the germination vigor, germination rate, germination index and vigor index were measured. And other indexes like the activities of nitrate reductase (NR), malondialdehyde (MDA), superoxide (SOD), peroxidase (POD) and catalase (CAT) were also measured.
The germination indexes of Perilla frutescens seeds under NaCl stress had obvious inhibition. But after the treatment with different concentrations of SNP, every germination indexes were all increased. And the seeds that treated with SNP with the concentration of 0.1 mmol/L has the most significantly increase in every index. The germination vigor was 60.4%, the germination rate was 78.3%, the germination index and vigor index were 13.7 and 0.1093 respectively. The content of MDA was decreased after the treatment of SNP. The activities of four enzymes included NR, SOD, POD and CAT were increased with the treatment of SNP, and get the maximin (1.52 microg/g x h, 0.32 U/mg, 5.9 U/mg and 4.8 U/mg respectively) with the concentration of 0.1 mmol/L SNP.
SNP with concentration of 0.1 mmol/L could significantly alleviate the damages to the seeds and seedlings of Perilla frutescens under NaCl stress, and promote the salt resistance of the seeds and seedlings.
探索提高紫苏种子和幼苗在NaCl胁迫下耐盐能力的方法。
测定了在NaCl胁迫下经硝普钠（SNP）处理的紫苏种子的发芽活力、发芽率、发芽指数和活力指数等生理指标。还测定了硝酸还原酶（NR）、丙二醛（MDA）、超氧化物歧化酶（SOD）、过氧化物酶（POD）和过氧化氢酶（CAT）等其他指标。
NaCl胁迫下紫苏种子的发芽指标受到明显抑制。但经不同浓度SNP处理后，各项发芽指标均有所提高。其中，0.1 mmol/L SNP处理的种子各项指标提高最为显著。发芽活力为60.4%，发芽率为78.3%，发芽指数和活力指数分别为13.7和0.1093。SNP处理后MDA含量降低。经SNP处理后，NR、SOD、POD和CAT这四种酶的活性均升高，且在0.1 mmol/L SNP浓度下达到最大值（分别为1.52 μg/g·h、0.32 U/mg、5.9 U/mg和4.8 U/mg）。
0.1 mmol/L的SNP能显著减轻NaCl胁迫对紫苏种子和幼苗的伤害，提高种子和幼苗的耐盐性。
