Zuidscherwoude Malou, Worah Kuntal, van der Schaaf Alie, Buschow Sonja I, van Spriel Annemiek B
Department of Tumor Immunology, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, Nijmegen, The Netherlands.
Centre for Molecular and Biomolecular Informatics (CMBI), Radboud University Medical Center, Nijmegen, the Netherlands.
PLoS One. 2017 Sep 7;12(9):e0184317. doi: 10.1371/journal.pone.0184317. eCollection 2017.
Dendritic cells (DCs), which are essential for initiating immune responses, are comprised of different subsets. Tetraspanins organize dendritic cell membranes by facilitating protein-protein interactions within the so called tetraspanin web. In this study we analyzed expression of the complete tetraspanin superfamily in primary murine (CD4+, CD8+, pDC) and human DC subsets (CD1c+, CD141+, pDC) at the transcriptome and proteome level. Different RNA and protein expression profiles for the tetraspanin genes across human and murine DC subsets were identified. Although RNA expression levels of CD37 and CD82 were not significantly different between human DC subsets, CD9 RNA was highly expressed in pDCs, while CD9 protein expression was lower. This indicates that relative RNA and protein expression levels are not always in agreement. Both murine CD8α+ DCs and its regarded human counterpart, CD141+ DCs, displayed relatively high protein levels of CD81. CD53 protein was highly expressed on human pDCs in contrast to the relatively low protein expression of most other tetraspanins. This study demonstrates that tetraspanins are differentially expressed by human and murine DC subsets which provides a valuable resource that will aid the understanding of tetraspanin function in DC biology.
树突状细胞（DCs）是启动免疫反应所必需的，由不同的亚群组成。四跨膜蛋白通过促进所谓的四跨膜蛋白网络内的蛋白质-蛋白质相互作用来组织树突状细胞膜。在本研究中，我们在转录组和蛋白质组水平分析了完整的四跨膜蛋白超家族在原代小鼠（CD4+、CD8+、浆细胞样DC）和人DC亚群（CD1c+、CD141+、浆细胞样DC）中的表达。确定了人源和鼠源DC亚群中四跨膜蛋白基因不同的RNA和蛋白质表达谱。虽然CD37和CD82的RNA表达水平在人DC亚群之间没有显著差异，但CD9 RNA在浆细胞样DC中高表达，而CD9蛋白表达较低。这表明相对的RNA和蛋白质表达水平并不总是一致的。小鼠CD8α+ DC及其对应的人源细胞CD141+ DC均显示出相对较高水平的CD81蛋白。与大多数其他四跨膜蛋白相对较低的蛋白表达相比，CD53蛋白在人浆细胞样DC上高表达。本研究表明，四跨膜蛋白在人源和鼠源DC亚群中差异表达，这为理解四跨膜蛋白在DC生物学中的功能提供了宝贵的资源。
