Morimoto Yasuo, Izumi Hiroto, Yoshiura Yukiko, Fujisawa Yuri, Yatera Kazuhiro, Fujita Katsuhide, Maru Junko, Endoh Shigehisa, Honda Kazumasa
University of Occupational and Environmental Health, Japan.
National Institute of Advanced Industrial Science and Technology, Japan.
Ind Health. 2018 Feb 7;56(1):30-39. doi: 10.2486/indhealth.2017-0082. Epub 2017 Sep 5.
In order to examine the usefulness of intratracheal instillation of nanoparticles for the screening of the harmful effects of nanoparticles, we performed intratracheal instillation studies of nanomaterials on rats using different delivery devices and postures as a basic study. Multiwall carbon nanotubes (MWCNTs) with a geometric mean length and secondary diameter of 2.16 μm and 752 nm, respectively, were used as the nanomaterials. Male F344 rats were intratracheally exposed to 0.04 or 0.2 mg/rat of MWCNT, were dissected at 1 d and 3 d, and cell analyses of the bronchoalveolar lavage fluid (BALF) were analyzed. Two delivery devices were used for the intratracheal instillation of the MWCNTs: a gavage needle and a microsprayer aerolizer. Both induced neutrophil influx in the lung at 1 and 3 d, and there were no significant differences in neutrophil inflammation between the two delivery devices. The main distribution of pulmonary inflammation by both delivery devices was in the centrilobular spaces in the lung. Two postures were used: an angle of approximately 45 degrees and a standing posture on a board, both of which also induced pulmonary influx in BALF and pulmonary inflammation mainly in the centrilobular spaces, with no large difference in pulmonary inflammation between the two postures. Taken together, the differences in the delivery devices and postures of the rats in the intratracheal instillation did not affect the acute pulmonary toxicity of the nanomaterials.
为了研究气管内滴注纳米颗粒用于筛选纳米颗粒有害效应的实用性，我们以不同的给药装置和姿势对大鼠进行气管内滴注纳米材料的研究作为基础研究。分别以几何平均长度和二次粒径为2.16μm和752nm的多壁碳纳米管（MWCNTs）作为纳米材料。将雄性F344大鼠经气管暴露于0.04或0.2mg/只的MWCNT，在1天和3天时进行解剖，并对支气管肺泡灌洗液（BALF）进行细胞分析。两种给药装置用于MWCNTs的气管内滴注：胃管针和微型喷雾器雾化器。两者在1天和3天时均诱导肺部中性粒细胞流入，且两种给药装置之间的中性粒细胞炎症无显著差异。两种给药装置引起的肺部炎症主要分布在肺小叶中心间隙。使用了两种姿势：大约45度角和在板上站立姿势，两者也均诱导BALF中的肺部流入以及主要在肺小叶中心间隙的肺部炎症，两种姿势之间的肺部炎症无大差异。综上所述，气管内滴注时大鼠的给药装置和姿势差异不影响纳米材料的急性肺毒性。
