基于凝胶的蛋白质组学分析中总蛋白染色的标准染料
Standard Dyes for Total Protein Staining in Gel-Based Proteomic Analysis.
作者信息
Chevalier François
机构信息
Proteomic Laboratory, iRCM, CEA, Fontenay aux Roses, France.
出版信息
Materials (Basel). 2010 Oct 20;3(10):4784-4792. doi: 10.3390/ma3104784.
Abstract
Staining of two-dimensional gels is a primary concern in proteomic studies using two-dimensional gel electrophoresis with respect to the number of proteins analyzed, the accuracy of spot quantification and reproducibility. In this review article, the efficiency of the most widely used dyes was investigated. Visible dyes (Coomassie blue and silver nitrate), fluorescent dyes (Sypro Ruby, Deep Purple) and cyanine labeled methods were compared.
摘要
在使用二维凝胶电泳的蛋白质组学研究中，二维凝胶的染色是一个主要关注点，涉及所分析蛋白质的数量、斑点定量的准确性和重现性。在这篇综述文章中，研究了最广泛使用的染料的效率。对可见染料（考马斯亮蓝和硝酸银）、荧光染料（Sypro Ruby、Deep Purple）和菁染料标记方法进行了比较。
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