Choudhary Suman, Kumaresan A, Kumar Manish, Chhillar Shivani, Malik Hrudananda, Kumar Sudarshan, Kaushik Jai K, Datta Tirtha K, Mohanty Ashok K
Animal Biotechnology Centre, National Dairy Research Institute, Karnal, Haryana 132001 India.
Livestock and Production Management Section, National Dairy Research Institute, Karnal, Haryana 132001 India.
J Anim Sci Biotechnol. 2017 Sep 1;8:69. doi: 10.1186/s40104-017-0201-5. eCollection 2017.
An oviduct- specific glycoprotein, OVGP1, is synthesized and secreted by non-ciliated epithelial cells of the mammalian oviduct which provides an essential milieu for reproductive functions. The present study reports the effects of recombinant buffalo OVGP1 that lacks post-translational modifications, and native Buffalo OVGP1 isolated from oviductal tissue, on frozen- thawed sperm functions and in vitro embryo development.
The proportion of viable sperms was greater ( < 0.05) in the recombinant OVGP1-treated group compared to the native OVGP1-treated group at 2 h, 3 h, and 4 h of incubation. The proportion of motile sperms at 3 h and 4 h of incubation; and membrane- intact sperms at 4 h was greater ( < 0.05) in the native OVGP1-treated group compared to the control and recombinant OVGP1-treated groups. The proportion of capacitated and acrosome- reacted sperms was greater ( < 0.05) in the native OVGP1-treated group compared to the recombinant OVGP1 group at 4 h. The rates of cleavage of embryos and their development to the blastocyst stage were greater ( < 0.05) in the presence of either native or recombinant OVGP1 in comparison to control at 10 μg/mL concentration as compared to 5 or 20 μg/mL.
The study suggests that both native and recombinant OVGP1 impart a positive effect on various sperm features and in vitro embryo development. However, native OVGP1 was found to have a more pronounced effect in comparison to recombinant non-glycosylated OVGP1 on various sperm functions except viability. Hence, our current findings infer that glycosylation of OVGP1 might be essential in sustaining the sperm functions but not the in vitro embryo development.
输卵管特异性糖蛋白OVGP1由哺乳动物输卵管的非纤毛上皮细胞合成并分泌，为生殖功能提供了必要的环境。本研究报告了缺乏翻译后修饰的重组水牛OVGP1以及从输卵管组织中分离出的天然水牛OVGP1对冻融精子功能和体外胚胎发育的影响。
在孵育2小时、3小时和4小时时，重组OVGP1处理组的活精子比例高于天然OVGP1处理组（P<0.05）。在孵育3小时和4小时时，天然OVGP1处理组的活动精子比例；以及在4小时时膜完整的精子比例均高于对照组和重组OVGP1处理组（P<0.05）。在4小时时，天然OVGP1处理组的获能和顶体反应精子比例高于重组OVGP1组（P<0.05）。与对照组相比，在10μg/mL浓度下，天然或重组OVGP1存在时胚胎的卵裂率及其发育到囊胚阶段的比例更高（P<0.05），而5μg/mL或20μg/mL时则不然。
该研究表明，天然和重组OVGP1对各种精子特征和体外胚胎发育均有积极影响。然而，与重组非糖基化OVGP1相比，天然OVGP1在除活力外的各种精子功能上具有更显著的作用。因此，我们目前的研究结果推断，OVGP1的糖基化可能对维持精子功能至关重要，但对体外胚胎发育并非如此。
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