Division of Strategic Research and Development, Graduate School of Science and Engineering, Satitama University, Saitama, Japan.
Shenzhen Key Laboratory for Orchid Conservation and Utilization, The National Orchid Conservation Center of China and The Orchid Conservation and Research Center of Shenzhen, Shenzhen, China, and The Center for Biotechnology and BioMedicine, Graduate School at Shenzhen, Tsinghua University, Shenzhen, China, and College of Arts, College of Landscape Architecture, Fujian Agriculture and Forestry University, Fuzhou, China.
Curr Issues Mol Biol. 2018;27:51-70. doi: 10.21775/cimb.027.051. Epub 2017 Sep 8.
Next-generation sequencing technologies are revolutionizing biology by permitting, transcriptome sequencing, whole-genome sequencing and resequencing, and genome-wide single nucleotide polymorphism profiling. Orchid research has benefited from this breakthrough, and a few orchid genomes are now available; new biological questions can be approached and new breeding strategies can be designed. The first part of this review describes the unique features of orchid biology. The second part provides an overview of the current next-generation sequencing platforms, many of which are already used in plant laboratories. The third part summarizes the state of orchid transcriptome and genome sequencing and illustrates current achievements. The genetic sequences currently obtained will not only provide a broad scope for the study of orchid biology, but also serves as a starting point for uncovering the mystery of orchid evolution.
下一代测序技术正在通过允许转录组测序、全基因组测序和重测序以及全基因组单核苷酸多态性分析来彻底改变生物学。兰花研究受益于这一突破，现在有几个兰花基因组可用；可以提出新的生物学问题并设计新的育种策略。本文的第一部分描述了兰花生物学的独特特征。第二部分概述了当前的下一代测序平台，其中许多已经在植物实验室中使用。第三部分总结了兰花转录组和基因组测序的现状，并说明了当前的成就。目前获得的遗传序列不仅将为兰花生物学的研究提供广泛的范围，而且还将作为揭示兰花进化奥秘的起点。
