Nakahama Naoyuki, Ito Azusa, Kaneko Shingo, Matsuki Yu, Suyama Yoshihisa, Hayano Azusa, Murayama Miho, Isagi Yuji
Graduate School of Arts and Sciences, The University of Tokyo.
Graduate School of Agriculture, Kyoto University.
Genes Genet Syst. 2018 Jul 13;93(1):31-35. doi: 10.1266/ggs.17-00021. Epub 2018 Jan 17.
Microsatellite markers were developed for the endangered orchid Calanthe izu-insularis (Orchidaceae). This species is unique to the Izu Islands in Japan. Unfortunately, its population size has decreased because of excessive collection for horticultural purposes. In addition, although natural hybridization between C. izu-insularis and C. discolor var. discolor has been reported, morphological differences between C. izu-insularis and the hybridized individuals remain unclear. Using next-generation sequencing, 11 polymorphic microsatellite markers were developed. All developed markers could amplify C. aristulifera and nine markers could amplify C. d. var. discolor, two other orchid species that are also endangered in Japan. The number of alleles and expected heterozygosity at each locus were 1-6 (mean, 2.35) and 0.00-0.79 (mean, 0.30), respectively. These microsatellite markers will help conservation geneticists in their investigation of the proportion of pure C. izu-insularis individuals in the Izu Islands.
为濒危兰花伊豆岛虾脊兰（兰科）开发了微卫星标记。该物种是日本伊豆群岛特有的。不幸的是，由于园艺用途的过度采集，其种群数量已经减少。此外，尽管已经报道了伊豆岛虾脊兰与异色虾脊兰变种异色虾脊兰之间的自然杂交，但伊豆岛虾脊兰与杂交个体之间的形态差异仍不清楚。利用下一代测序技术，开发了11个多态性微卫星标记。所有开发的标记都能扩增有喙虾脊兰，9个标记能扩增异色虾脊兰变种异色虾脊兰，这是另外两种在日本也濒危的兰花物种。每个位点的等位基因数和预期杂合度分别为1 - 6（平均2.35）和0.00 - 0.79（平均0.30）。这些微卫星标记将有助于保护遗传学家调查伊豆群岛上纯伊豆岛虾脊兰个体的比例。
