Ganji Nirmala, Chityala Vijay Kumar, Marri Pradeep Kumar, Aveli Rambabu, Narendrula Vamsikrishna, Daravath Sreenu
Department of Chemistry, Osmania University, Hyderabad, Telangana 500007, India.
Department of Chemistry, Osmania University, Hyderabad, Telangana 500007, India.
J Photochem Photobiol B. 2017 Oct;175:132-140. doi: 10.1016/j.jphotobiol.2017.08.038. Epub 2017 Aug 31.
Two new series of binary metal complexes [M(L)] and [M(L)] where, M=Cu(II), Ni(II) & Co(II) and L=4-((3,4-dimethylisoxazol-5-ylimino)methyl)benzene-1,3-diol; L=2-((3,4-dimethylisoxazol-5-ylimino)methyl)-5-methoxyphenol were synthesized and characterized by elemental analysis, H NMR, C NMR, FT-IR, ESI mass, UV-Visible, magnetic moment, ESR, SEM and powder XRD studies. Based on these results, a square planar geometry is assigned for all the metal complexes where the Schiff base acts as uninegatively charged bidentate chelating agent via the hydroxyl oxygen and azomethine nitrogen atoms. DNA binding studies of all the complexes with calf thymus DNA have been comprehensively investigated using electronic absorption spectroscopy, fluorescence quenching and viscosity studies. The oxidative and photo cleavage affinity of metal complexes towards supercoiled pBR322 DNA has been ascertained by agarose gel electrophoresis assay. From the results, it is observed that all the metal complexes bind effectively to CT-DNA via an intercalative mode of binding and also cleave pBR322 DNA in a promising manner. Further the Cu(II) complexes have shown better binding and cleavage properties towards DNA. The antimicrobial activities of the Schiff bases and their metal complexes were studied on bacterial and fungal strains and the results denoted that the complexes are more potent than their Schiff base ligands.
合成了两个新的二元金属配合物系列[M(L)]和[M(L)]，其中M = Cu(II)、Ni(II)和Co(II)，L = 4-((3,4-二甲基异恶唑-5-基亚氨基)甲基)苯-1,3-二醇；L = 2-((3,4-二甲基异恶唑-5-基亚氨基)甲基)-5-甲氧基苯酚，并通过元素分析、氢核磁共振、碳核磁共振、傅里叶变换红外光谱、电喷雾质谱、紫外可见光谱、磁矩、电子自旋共振、扫描电子显微镜和粉末X射线衍射研究对其进行了表征。基于这些结果，为所有金属配合物指定了平面正方形几何构型，其中席夫碱通过羟基氧原子和偶氮甲碱氮原子作为单负电荷双齿螯合剂。使用电子吸收光谱、荧光猝灭和粘度研究全面研究了所有配合物与小牛胸腺DNA的DNA结合研究。通过琼脂糖凝胶电泳测定确定了金属配合物对超螺旋pBR322 DNA的氧化和光裂解亲和力。从结果可以看出，所有金属配合物都通过插入结合模式有效地与CT-DNA结合，并且还以有前景的方式切割pBR322 DNA。此外，Cu(II)配合物对DNA表现出更好的结合和切割特性。研究了席夫碱及其金属配合物对细菌和真菌菌株的抗菌活性，结果表明配合物比其席夫碱配体更有效。
