Department of Cardiothoracic Surgery, Clinical College of Yangzhou University, Yangzhou, 225001, China.
Key Laboratory of Integrative Medicine in Geriatrics Control of Jiangsu Province, Yangzhou University, Yangzhou, 225001, China.
Bioprocess Biosyst Eng. 2017 Dec;40(12):1813-1823. doi: 10.1007/s00449-017-1835-6. Epub 2017 Sep 8.
The goals of our study were to evaluate the biomechanical properties and cellular biocompatibility of 3D printed tracheal graft fabricated by polycaprolactone (PCL). Compared with native tracheal patch, there was a significant increase in maximum stress and elastic modulus for 3DP tracheal graft (p < 0.05). BMSCs were co-cultured under four different conditions to investigate cytotoxicity of the graft: (1) co-cultured with normal culture medium, as blank control; (2) co-cultured with perfluoropropylene, as negative control; (3) co-cultured with 3DP tracheal graft; and (4) co-cultured with polyvinyl chloride, as positive control. Moreover, the results of SRB assay showed that compared with blank and negative control group, there was no significant difference in the cell proliferation of 3DP tracheal graft group for 21 days (p > 0.05). These results revealed that 3DP tracheal graft in our study has favorable cellular biocompatibility and biomechanical properties, and, therefore, will be a promising alternative for tissue-engineered trachea.
我们的研究目的是评估由聚己内酯（PCL）制造的 3D 打印气管移植物的生物力学特性和细胞生物相容性。与天然气管补片相比，3DP 气管移植物的最大应力和弹性模量显著增加（p<0.05）。将 BMSCs 在四种不同条件下共培养，以研究移植物的细胞毒性：（1）与正常培养基共培养，作为空白对照；（2）与全氟丙烷共培养，作为阴性对照；（3）与 3DP 气管移植物共培养；（4）与聚氯乙烯共培养，作为阳性对照。此外，SRB 检测结果表明，与空白对照组和阴性对照组相比，3DP 气管移植物组在 21 天内的细胞增殖无显著差异（p>0.05）。这些结果表明，我们研究中的 3DP 气管移植物具有良好的细胞生物相容性和生物力学性能，因此将成为组织工程气管的一种有前途的替代品。
