铅通过Fas/FasL死亡受体途径诱导小鼠TM3 Leydig细胞凋亡。
Lead induces apoptosis in mouse TM3 Leydig cells through the Fas/FasL death receptor pathway.
作者信息
He Xiuyuan, Wu Jing, Yuan Liyun, Lin Feng, Yi Jine, Li Jing, Yuan Hui, Shi Jinling, Yuan Tingting, Zhang Shufang, Fan Yongheng, Zhao Zhihang
机构信息
College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan, 450002, China.
College of Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan, 410128, China.
出版信息
Environ Toxicol Pharmacol. 2017 Dec;56:99-105. doi: 10.1016/j.etap.2017.08.034. Epub 2017 Sep 1.
The study was aimed to investigate the effect of Pb toxicity on mouse Leydig cells and its molecular mechanism. The TM3 cells were cultured in vitro and exposed to Pb at different concentrations for 24h. The effects of Pb on cell proliferation and apoptosis were analyzed with MTT and Annexin V-FITC/PI via flow cytometry, respectively. Expression levels of Fas, Fas-L and caspase-8 in TM3 cells were determined by western blot. As well as the inhibitory effect of the caspase-8 inhibitor Z-IETD-FMK on cell apoptosis. We found that Pb treatment significantly decreased the cellar viability (P<0.05), increased the apoptosis (P<0.01) and the Fas, FasL, and caspase-8 expression levels in Pb-treated cells as compared to the control cells (P<0.05 or P<0.01). Furthermore, the caspase-8 inhibitor effectively block the Pb-induced cell apoptosis. Taken together, our data suggest that Pb-induced TM3 cell toxic effect may involve in the Fas/FasL death receptor signaling pathway.
本研究旨在探讨铅毒性对小鼠睾丸间质细胞的影响及其分子机制。将TM3细胞进行体外培养，并暴露于不同浓度的铅中24小时。分别采用MTT法和Annexin V-FITC/PI通过流式细胞术分析铅对细胞增殖和凋亡的影响。通过蛋白质免疫印迹法测定TM3细胞中Fas、Fas-L和caspase-8的表达水平。以及caspase-8抑制剂Z-IETD-FMK对细胞凋亡的抑制作用。我们发现，与对照细胞相比，铅处理显著降低了细胞活力（P<0.05），增加了凋亡（P<0.01）以及铅处理细胞中Fas、FasL和caspase-8的表达水平（P<0.05或P<0.01）。此外，caspase-8抑制剂有效地阻断了铅诱导的细胞凋亡。综上所述，我们的数据表明，铅诱导的TM3细胞毒性作用可能涉及Fas/FasL死亡受体信号通路。
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