Doras Camille, Petak Ferenc, Bayat Sam, Baudat Aurélie, Von Garnier Christophe, Eigenmann Philippe, Habre Walid
Anaesthesiological Investigations Unit, University Hospitals of Geneva, Geneva, Switzerland.
Department of Medical Physics and Informatics, University of Szeged, Szeged, Hungary.
Respir Physiol Neurobiol. 2018 Jan;247:43-51. doi: 10.1016/j.resp.2017.09.001. Epub 2017 Sep 8.
Ovalbumin (OVA) sensitization has limitations in modelling asthma. Thus, we examined the value of allergic sensitization using a purified natural allergen, house dust mite (HDM), over the sensitization performed with OVA. Mice were sham-treated, or sensitized with OVA- or HDM with identical chronology. Airway resistance, tissue damping and elastance were assessed under control conditions and after challenging the animals with methacholine (MCh) and the specific allergen. Inflammatory profile of the bronchoalveolar lavage fluid was characterized and lung histology was performed. While no difference in the lung responsiveness to the specific allergen was noted, hyperresponsiveness to MCh was observed only in the HDM-sensitized animals in the lung peripheral parameters. Lung inflammation differed between the models, but excessive bronchial smooth muscle remodelling occurred only with OVA. In conclusion, we demonstrate that a purified natural allergen offers a more relevant murine model of human allergic asthma by expressing the key features of this chronic inflammatory disease both in the lung function and structure.
卵清蛋白（OVA）致敏在哮喘建模方面存在局限性。因此，我们研究了使用纯化的天然过敏原屋尘螨（HDM）进行过敏致敏相对于用OVA进行致敏的价值。对小鼠进行假处理，或以相同的时间顺序用OVA或HDM进行致敏。在对照条件下以及用乙酰甲胆碱（MCh）和特异性过敏原对动物进行激发后，评估气道阻力、组织阻尼和弹性。对支气管肺泡灌洗液的炎症特征进行了表征，并进行了肺组织学检查。虽然未观察到肺对特异性过敏原的反应性存在差异，但仅在HDM致敏的动物中，在肺外周参数方面观察到对MCh的高反应性。两种模型的肺部炎症有所不同，但仅OVA致敏会导致过度的支气管平滑肌重塑。总之，我们证明，一种纯化的天然过敏原通过在肺功能和结构方面表达这种慢性炎症性疾病的关键特征，提供了一个更符合人类过敏性哮喘的小鼠模型。
