a College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling 712100, China.
b Biological Science Department, Dezhou University, Dezhou, Shandong 253023, China.
Genome. 2017 Oct;60(10):837-849. doi: 10.1139/gen-2017-0033. Epub 2017 Sep 11.
Accumulating evidence indicates that Krüppel-like factors (KLFs) play important roles in fat biology via the regulation of CCAAT/enhancer binding proteins (C/EBPs). However, KLFs and C/EBPs have not been identified from Strongylocentrotus nudus, and their roles in this species are not clear. In this study, the full-length cDNA of S. nudus KLF10 (SnKLF10) and three cDNA fragments of S. nudus C/EBPs (SnC/EBPs) were obtained. Examination of tissue distribution and expression patterns during gonadal development implied that SnKLF10 and SnC/EBPs play important roles in gonadal lipogenesis. The presence of transcription factor-binding sites (TFBSs) for KLFs in SnC/EBPs, and the results of an over-expression assay, revealed that SnKLF10 negatively regulates the transcription of SnC/EBPs. In addition, the core promoter regions of SnC/EBPs were determined, and multiple TFBSs for transcription factor (TFs) were identified, which are potential regulators of SnC/EBP transcription. Taken together, these results suggest that SnC/EBP genes are potential targets of SnKLF10, and that SnKLF10 plays a role in lipogenesis by repressing the transcription of SnC/EBPs. These findings provide information for further studies of KLF10 in invertebrates and provide new insight into the regulatory mechanisms of C/EBP transcription.
越来越多的证据表明，Krüppel 样因子（KLFs）通过调节 CCAAT/增强子结合蛋白（C/EBPs）在脂肪生物学中发挥重要作用。然而，KLFs 和 C/EBPs 尚未在海参中被鉴定出来，它们在该物种中的作用尚不清楚。在这项研究中，获得了海参 KLF10（SnKLF10）的全长 cDNA 和三个海参 C/EBPs（SnC/EBPs）的 cDNA 片段。组织分布和性腺发育过程中的表达模式研究表明，SnKLF10 和 SnC/EBPs 在性腺脂肪生成中发挥重要作用。SnC/EBPs 中存在 KLFs 的转录因子结合位点（TFBSs），以及过表达试验的结果表明，SnKLF10 负调控 SnC/EBPs 的转录。此外，确定了 SnC/EBPs 的核心启动子区域，并鉴定出多个转录因子（TFs）的 TFBSs，它们可能是 SnC/EBP 转录的调节剂。综上所述，这些结果表明，SnC/EBP 基因是 SnKLF10 的潜在靶标，SnKLF10 通过抑制 SnC/EBPs 的转录在脂肪生成中发挥作用。这些发现为进一步研究无脊椎动物中的 KLF10 提供了信息，并为 C/EBP 转录的调控机制提供了新的见解。
