蜘蛛毒素可阻断离体海马锥体神经元中兴奋性氨基酸反应。

Spider toxin blocks excitatory amino acid responses in isolated hippocampal pyramidal neurons.

作者信息

Akaike N, Kawai N, Kiskin N I, Kljuchko E M, Krishtal O A

机构信息

A.A. Bogomoletz Institute of Physiology, Ukrainian Academy of Science, Kiev, (U.S.S.R.).

出版信息

Neurosci Lett. 1987 Aug 31;79(3):326-30. doi: 10.1016/0304-3940(87)90453-8.

DOI:10.1016/0304-3940(87)90453-8

PMID:2889174

Abstract

Using the 'concentration-clamp' technique we have investigated the action of Joro spider toxin (JSTX), as a specific blocker of glutamate receptor, on freshly isolated rat hippocampal pyramidal neurons. The neurons showed prominent responses to L-glutamate (L-Glu), quisqualate (QA) and kainate (KA) and JSTX blocked completely the both responses. The blocking action of toxin was dose-dependent at the concentrations of toxin between 4.8 X 10(-12) and 4.8 X 10(-8) M, and was remarkably similar in cell to cell trials. The kinetics of blockade was revealed by applying the toxin to the non-desensitizing KA response. We suggest that the JSTX spider toxin may be a valuable tool in ligand binding studies of QA/KA receptors in the central nervous system.

摘要

我们运用“浓度钳制”技术，研究了作为谷氨酸受体特异性阻断剂的雀瓢蛛毒素（JSTX）对新鲜分离的大鼠海马锥体神经元的作用。这些神经元对L-谷氨酸（L-Glu）、quisqualate（QA）和海人藻酸（KA）表现出显著反应，而JSTX完全阻断了这两种反应。在毒素浓度为4.8×10⁻¹²至4.8×10⁻⁸ M之间时，毒素的阻断作用呈剂量依赖性，并且在细胞间试验中非常相似。通过将毒素应用于非脱敏性KA反应来揭示阻断动力学。我们认为，JSTX蜘蛛毒素可能是中枢神经系统中QA/KA受体配体结合研究的一种有价值的工具。