培养的小鼠脊髓神经元上的阿片肽受体类型。

Opiate peptide receptor types on cultured mouse spinal neurons.

作者信息

Jia M, Nelson P G

机构信息

Laboratory of Developmental Neurobiology, National Institute of Child Health and Human Development, Bethesda, MD 20892.

出版信息

Peptides. 1987 May-Jun;8(3):559-63. doi: 10.1016/0196-9781(87)90023-4.

DOI:10.1016/0196-9781(87)90023-4

PMID:2889202

Abstract

mu, delta and kappa opioid receptor agonists, morphiceptin, Leu-enkephalin and dynorphin reduced monosynaptic EPSPs evoked in spinal cord neurons by stimulation of spinal cord neurons in a mouse cell culture system. The incidence of the cell pairs which responded to morphiceptin, Leu-enkephalin and dynorphin was 3%, 63% and 37% respectively. Statistical analysis showed the effect of Leu-enkephalin was presynaptic. When tested with Leu-enkephalin and dynorphin, 6 cell pairs responded to both Leu-enkephalin and dynorphin, 5 cell pairs only responded to Leu-enkephalin, none of the cell pairs responded only to dynorphin (n = 18). It is suggested that some cells have only delta receptors, but kappa receptors coexist with delta receptors. Opiate receptors of the mu type are rare on SC neurons.

摘要

μ、δ和κ阿片受体激动剂、吗啡肽、亮氨酸脑啡肽和强啡肽在小鼠细胞培养系统中，通过刺激脊髓神经元，减少了脊髓神经元中诱发的单突触兴奋性突触后电位（EPSP）。对吗啡肽、亮氨酸脑啡肽和强啡肽有反应的细胞对的发生率分别为3%、63%和37%。统计分析表明亮氨酸脑啡肽的作用是突触前的。当用亮氨酸脑啡肽和强啡肽进行测试时，6个细胞对同时对亮氨酸脑啡肽和强啡肽有反应，5个细胞对仅对亮氨酸脑啡肽有反应，没有细胞对仅对强啡肽有反应（n = 18）。这表明一些细胞只有δ受体，但κ受体与δ受体共存。μ型阿片受体在脊髓神经元上很少见。