de la Calle Maria B, Devesa Vicenta, Fiamegos Yiannis, Vélez Dinoraz
European Commission, Joint Research Centre;
Metal Trace Element Laboratory, IATA-CSIC.
J Vis Exp. 2017 Sep 1(127):55953. doi: 10.3791/55953.
The European Food Safety Authority (EFSA) underlined in its Scientific Opinion on Arsenic in Food that in order to support a sound exposure assessment to inorganic arsenic through diet, information about distribution of arsenic species in various food types must be generated. A method, previously validated in a collaborative trial, has been applied to determine inorganic arsenic in a wide variety of food matrices, covering grains, mushrooms and food of marine origin (31 samples in total). The method is based on detection by flow injection-hydride generation-atomic absorption spectrometry of the iAs selectively extracted into chloroform after digestion of the proteins with concentrated HCl. The method is characterized by a limit of quantification of 10 µg/kg dry weight, which allowed quantification of inorganic arsenic in a large amount of food matrices. Information is provided about performance scores given to results obtained with this method and which were reported by different laboratories in several proficiency tests. The percentage of satisfactory results obtained with the discussed method is higher than that of the results obtained with other analytical approaches.
欧洲食品安全局（EFSA）在其关于食品中砷的科学意见中强调，为了支持通过饮食对无机砷进行合理的暴露评估，必须获取各种食品类型中砷形态分布的信息。一种先前在合作试验中得到验证的方法已被用于测定多种食品基质中的无机砷，这些基质包括谷物、蘑菇和海洋来源的食品（总共31个样本）。该方法基于在使用浓盐酸消化蛋白质后，通过流动注射-氢化物发生-原子吸收光谱法检测选择性萃取到氯仿中的无机砷。该方法的定量限为10 µg/kg干重，这使得能够对大量食品基质中的无机砷进行定量。文中提供了关于该方法所得结果的性能评分信息，这些结果由不同实验室在多次能力验证试验中报告。所讨论方法获得的满意结果百分比高于其他分析方法所得结果的百分比。
