Singh Gurjeet, Urhekar A D
Assistant Professor, Department of Microbiology, N. C. Medical College and Hospital, Panipat, Haryana, India.
Professor and Head, Department of Microbiology, MGM Medical College and Hospital, Navi Mumbai, Maharashtra, India.
J Clin Diagn Res. 2017 Jul;11(7):DC13-DC18. doi: 10.7860/JCDR/2017/24055.10211. Epub 2017 Jul 1.
Pathogenesis of aspergillosis is dependent on various factors of the host (immune status) and virulence factors of the pathogen which could play a significant role in the pathogenesis of invasive aspergillosis.
To study the virulence factors of species isolated from patient samples and environmental samples.
This prospective and experimental study was carried out at Department of Microbiology, MGM Medical College and Hospital, Mumbai, Maharashtra, India, from July 2014 to June 2015. For detection of virulence factors of species, total 750 samples were included in this study (350 from patients and 400 samples from environment). Patient samples and hospital environment samples were subjected to standard methods for screening of Biofilm, Lipase, α-amylase, proteinase, haemolysin, phospholipase and pectinase. Statistical analysis was done using Chi-square test and SPSS (Version 17.0).
American Type Culture Collection (ATCC) control of and showed production of all virulence factors. In patient samples maximum virulence factor was produced i.e., α-amylase activity (89.74%) followed by proteinase activity (87.17%), biofilm production was (82.05%) haemolysin activity (79.48%), lipase activity (66.66%), pectinase activity and phospholipase activity (61.53%). In environment samples maximum virulence factor was produced i.e., proteinase activity (41.02%) followed by biofilm production was (38.46%), α-amylase activity (35.89%), haemolysin activity (33.33%), lipase activity (28.20%), phospholipase (25.64%) and pectinase activity (23.07%). The differences in patient and environment virulence factors were statistically significant (p-value <0.05).
Overall the presence of virulence factors was found more in species isolated from patient samples then environmental samples. This could be due to invasiveness nature of Aspergilli. was common isolates from both patient and environmental samples. Our study highlights the possible transmission of Aspergilli from environment to patient. Detection of virulence factors of species help to differentiate between pathogenic and non-pathogenic Aspergilli. Presence of virulence factors confirmed pathogenicity of the isolates. It also helps the physicians to treat the patient when appropriate treatment is needed.
曲霉病的发病机制取决于宿主的多种因素（免疫状态）以及病原体的毒力因子，这些因素在侵袭性曲霉病的发病机制中可能起重要作用。
研究从患者样本和环境样本中分离出的菌种的毒力因子。
本前瞻性实验研究于2014年7月至2015年6月在印度马哈拉施特拉邦孟买的MGM医学院和医院微生物科进行。为检测菌种的毒力因子，本研究共纳入750个样本（350个来自患者，400个来自环境）。患者样本和医院环境样本采用标准方法进行生物膜、脂肪酶、α淀粉酶、蛋白酶、溶血素、磷脂酶和果胶酶的筛查。使用卡方检验和SPSS（版本17.0）进行统计分析。
美国典型培养物保藏中心（ATCC）的 和 的对照显示产生了所有毒力因子。在患者样本中，产生的毒力因子最多的是α淀粉酶活性（89.74%），其次是蛋白酶活性（87.17%）、生物膜产生（82.05%）、溶血素活性（79.48%）、脂肪酶活性（66.66%）、果胶酶活性和磷脂酶活性（61.53%）。在环境样本中，产生的毒力因子最多的是蛋白酶活性（41.02%），其次是生物膜产生（38.46%）、α淀粉酶活性（35.89%）、溶血素活性（33.33%）、脂肪酶活性（28.20%）、磷脂酶（25.64%）和果胶酶活性（23.07%）。患者和环境毒力因子的差异具有统计学意义（p值<0.05）。
总体而言，从患者样本中分离出的菌种比从环境样本中分离出的菌种中发现的毒力因子更多。这可能是由于曲霉的侵袭性。 是患者和环境样本中常见的分离株。我们的研究强调了曲霉从环境传播到患者的可能性。检测菌种的毒力因子有助于区分致病性和非致病性曲霉。毒力因子的存在证实了分离株的致病性。当需要适当治疗时，它也有助于医生治疗患者。
