Katiyar S K, Prasad A K, Ghoshal S, Das S R, Sagar P
Microbiology Division, Central Drug Research Institute, Lucknow, India.
Ann Trop Med Parasitol. 1987 Jun;81(3):201-5. doi: 10.1080/00034983.1987.11812113.
Repeated passage of the 200-NIH strain of Entamoeba histolytica through cholesterol-enriched axenic growth medium induced marked increases in cholesterol, phosphoglucomutase and hexokinase levels and a less prominent rise in the protein content of amoebic cells. There was also pronounced enhancement of haemolytic activity and Concanavalin A (Con A) agglutinability of the culture, but no significant change was observed in glucose phosphate isomerase. These cholesterol-induced effects persisted to a large extent when amoebae were subsequently repassaged through normal axenic medium lacking exogenous cholesterol, but changes in cellular cholesterol and protein levels did not persist. Qualitatively similar results were obtained whether the sterol was layered as a film on the glass walls of the culture tubes or supplied as sonicated micells, but the latter was in general more effective.
将溶组织内阿米巴200 - NIH株反复传代于富含胆固醇的无细菌生长培养基中，可使胆固醇、磷酸葡萄糖变位酶和己糖激酶水平显著升高，阿米巴细胞蛋白质含量升高幅度较小。培养物的溶血活性和刀豆球蛋白A（Con A）凝集性也有明显增强，但磷酸葡萄糖异构酶未观察到显著变化。当随后将阿米巴转种于缺乏外源性胆固醇的正常无细菌培养基中时，这些胆固醇诱导的效应在很大程度上仍然存在，但细胞胆固醇和蛋白质水平的变化并未持续。无论固醇是以薄膜形式铺在培养管玻璃壁上还是以超声处理的微团形式提供，都获得了定性相似的结果，但后者一般更有效。
