Houshmand Massoud, Abbaszadegan Mohammad Reza, Kerachian Mohammad Amin
Department of Medical Genetics, National Institute for Genetic Engineering and Biotechnology, Tehran, Iran.
Medical Genetics Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
Middle East J Dig Dis. 2017 Jul;9(3):158-163. doi: 10.15171/mejdd.2017.67.
BACKGROUND Colorectal cancer (CRC) is a common cancer that results in outstanding morbidity and mortality worldwide. DNA methylation is one of the most important epigenetic events that is thought to occur during the early stages of oncogenic transformation especially in CRC. The aim of this study was to investigate whether hypermethylation of bone morphogenetic protein 3 (BMP3) in tissue samples is implicated in Iranian patients with CRC. METHODS From fresh frozen tissue samples of 30 patients with CRC, the DNA was isolated, treated with sodium bisulfite and analyzed by methylation-specific polymerase chain reaction with primers specific for methylated or unmethylated promoter sequences of the BMP3 gene. Demographic characteristics of the patients including age, sex, tumor grade, location, stage, and TNM classification were evaluated and the relationship between hypermethylation of the gene and clinicopathological features was analyzed. RESULTS Methylation of the BMP3 promoter was often present in the DNA extracted from the tumoral tissues. A sensitivity of 56.66% and specificity of 93.3% were attained in the detection of colorectal neoplasia. CONCLUSION We assumed that solely BMP3 methylation analysis in our population is not sufficient to select the gene as a screening biomarker and it should be considered in combination with other markers to screen for detection of colorectal cancer.
结直肠癌（CRC）是一种常见癌症，在全球范围内导致显著的发病率和死亡率。DNA甲基化是最重要的表观遗传事件之一，被认为发生在致癌转化的早期阶段，尤其是在结直肠癌中。本研究的目的是调查组织样本中骨形态发生蛋白3（BMP3）的高甲基化是否与伊朗结直肠癌患者有关。方法：从30例结直肠癌患者的新鲜冷冻组织样本中分离DNA，用亚硫酸氢钠处理，并通过甲基化特异性聚合酶链反应，使用针对BMP3基因甲基化或未甲基化启动子序列的特异性引物进行分析。评估患者的人口统计学特征，包括年龄、性别、肿瘤分级、位置、分期和TNM分类，并分析基因高甲基化与临床病理特征之间的关系。结果：BMP3启动子的甲基化经常出现在从肿瘤组织中提取的DNA中。在检测结直肠肿瘤时，灵敏度达到56.66%，特异性达到93.3%。结论：我们认为，仅对我们人群中的BMP3甲基化进行分析不足以将该基因选为筛查生物标志物，应结合其他标志物来筛查结直肠癌的检测。
