胰蛋白酶原激活肽诱导大鼠胰腺腺泡细胞释放高迁移率族蛋白B1

Trypsinogen Activation Peptide Induces HMGB1 Release from Rat Pancreatic Acinar Cells.

作者信息

Wang Guoliang, Liu Yan, Dui Danhua, Bai Liang, Liu Yao, Tian Fei, Wei Wei

机构信息

Department of Hepatobiliaropancreatic Surgery, Guizhou Provincial People's Hospital, Guiyang, 550002, China.

Department of Hepatopancreatobiliary Surgery, Affiliated Hospital of Zunyi Medical, College, Zunyi563003, China.

出版信息

Open Med (Wars). 2017 Sep 6;12:293-298. doi: 10.1515/med-2017-0043. eCollection 2017.

DOI:10.1515/med-2017-0043

PMID:28894846

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5588754/

Abstract

BACKGROUND

The development of acute pancreatitis (AP) is associated with intracellular events in pancreatic cells, as well as with early and late inflammatory responses; however, their underlying mechanisms remain unclear. This study investigated trypsinogen activation peptide (TAP)-induced release of high mobility group box-l (HMGB1) from pancreatic acinar cells and how ethyl pyruvate (EP) affects this release.

METHODOLOGY

Pancreatic acinar cells from Sprague Dawley rats were divided into control, TAP (administered TAP), and EP (administered TAP and EP) groups. Cells were collected at 3, 6, 12, and 24 hours after TAP administration to detect HMGB1 mRNA and protein levels using quantitative PCR (qPCR) and Western blotting, respectively.

RESULTS

The TAP and EP groups exhibited higher levels of HMGB1 mRNA and protein expression (P<0.05) than the control group. The HMGB1 mRNA and protein expression levels also increased with prolonged TAP activity (P<0.05)-especially at 12 and 24 hours (P<0.01)-and showed positive correlations with TAP activity duration (3, 6, 12, and 24 hours) (r=0.971, P<0.01; r=0.966, P<0.01, respectively).

CONCLUSION

TAP induces HMGB1 release from pancreatic acinar cells. A positive temporal link exists between early TAP activity and late HMGB1 expression in AP, and EP inhibits HMGB1 release.

摘要

背景

急性胰腺炎（AP）的发展与胰腺细胞内的事件以及早期和晚期炎症反应相关；然而，其潜在机制仍不清楚。本研究调查了胰蛋白酶原激活肽（TAP）诱导胰腺腺泡细胞释放高迁移率族蛋白B1（HMGB1）的情况，以及丙酮酸乙酯（EP）如何影响这种释放。

方法

将来自Sprague Dawley大鼠的胰腺腺泡细胞分为对照组、TAP组（给予TAP）和EP组（给予TAP和EP）。在给予TAP后3、6、12和24小时收集细胞，分别使用定量PCR（qPCR）和蛋白质免疫印迹法检测HMGB1 mRNA和蛋白质水平。

结果

TAP组和EP组的HMGB1 mRNA和蛋白质表达水平高于对照组（P<0.05）。随着TAP活性时间延长，HMGB1 mRNA和蛋白质表达水平也升高（P<0.05），尤其是在12和24小时（P<0.01），并且与TAP活性持续时间（3、6、12和24小时）呈正相关（r分别为0.971，P<0.01；r为0.966，P<0.01）。