Woodyard Ethan T, Rosser Thomas Graham, Rush Scott A
Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, MS, 39762, USA.
Department of Wildlife, Fisheries, and Aquaculture, College of Forest Resources, Mississippi State University, Mississippi State, MS, 39762, USA.
Parasitol Res. 2017 Nov;116(11):2981-2993. doi: 10.1007/s00436-017-5607-7. Epub 2017 Sep 11.
Based on specimens collected from harvested American alligator Alligator mississippiensis Daudin, 1801 in Mississippi, USA, novel molecular data for both nuclear ribosomal genes (18S, ITS1-5.8S, ITS2, and 28S) and mitochondrial genes (cytochrome c oxidase subunit 1 and nicotinamide adenine dinucleotide dehydrogenase subunit 1) are provided for Odhneriotrema incommodum (Leidy, 1856), a trematode of the family Clinostomidae Lühe, 1901 infecting A. mississippiensis and the Florida spotted gar Lepisosteus platyrhincus DeKay, 1842. This represents the first sequencing data available for the genus Odhneriotrema and the subfamily Nephrocephalinae Travassos, 1928. Additionally, the results of phylogenetic analyses, additional morphometric data, a photomicrograph, and a line drawing supporting the present identification of O. incommodum are provided. These data will aid in elucidating the life cycle of O. incommodum through molecular identification of larval stages as well as understanding the evolutionary history of Clinostomidae and its subfamilies. Implications for the currently accepted organization of the Clinostomidae are discussed.
基于从美国密西西比州捕获的1801年密西西比鳄（Alligator mississippiensis Daudin）采集的标本，提供了关于不适奥氏吸虫（Odhneriotrema incommodum，Leidy，1856）的核糖体基因（18S、ITS1 - 5.8S、ITS2和28S）和线粒体基因（细胞色素c氧化酶亚基1和烟酰胺腺嘌呤二核苷酸脱氢酶亚基1）的新分子数据，该吸虫属于1901年吕赫（Lühe）的嗜眼科（Clinostomidae），感染密西西比鳄和1842年德凯（DeKay）的佛罗里达雀鳝（Lepisosteus platyrhincus）。这代表了不适奥氏吸虫属和1928年特拉瓦索斯（Travassos）的肾头亚科（Nephrocephalinae）的首个测序数据。此外，还提供了系统发育分析结果、额外的形态测量数据、一张显微照片和一幅线条图，以支持当前对不适奥氏吸虫的鉴定。这些数据将有助于通过幼虫阶段的分子鉴定阐明不适奥氏吸虫的生命周期，以及了解嗜眼科及其亚科的进化历史。讨论了对当前接受的嗜眼科分类的影响。
