Wang Shi, Dong Xian-Zhe, Tan Xiao, Wang Yu-Ning, Liu Ping
Department of Clinical Pharmacology, General Hospital of PLA, Beijing 100853, China.
Zhongguo Zhong Yao Za Zhi. 2016 May;41(10):1898-1902. doi: 10.4268/cjcmm20161023.
To observe the effect of antidepressant medicine prescription, Kaixinsan (KXS) on monoamine oxidase (MAO) activity, and explore the mechanism of KXS in elevating the levels of monoamine neurotransmitter from the perspective of metabolism, in vitro enzyme reaction system and C6 neuroglial cells, the effect of KXS at different concentrations on MAO-A and MAO-B activity was observed. In animal studies, the effect of KXS at different concentrations on MAO-A and MAO-B activities of brain mitochondrialin normal rats and solitary chronic unpredictable moderate stress (CMS) model rats after intragastric administration for 1, 2, 3 weeks. Results showed that 10 g•L⁻¹ KXS could significantly reduce the activity of MAO-A and MAO-B in enzyme reaction system; and in C6 cells, KXS within 0.625-10 g•L⁻¹ concentration range had no significant effect on the activity of MAO-A, but had obvious inhibitory effect on the activity of MAO-B in a dose dependent manner. KXS had no significant effect on the activity of MAO-A and MAO-B in brains of normal rats after action for 1, 2, 3 weeks. After 2 and 3 weeks treatment with 338 mg•kg⁻¹ dose KXS, MAO-A activity in the brain of CMS rats was decreased as compared with the model group (P<0.05), while KXS had no significant effect on MAO-B activity after 1, 2, 3 weeks of treatment. The results indicated that KXS had certain effect on in vitro MAO-A and MAO-B activity, had no effect on brain MAO-A and MAO-B activity in vivo in normal rats, and had certain inhibitory effect on MAO-A activity in brains of CMS rats.
为观察抗抑郁药开心散（KXS）对单胺氧化酶（MAO）活性的影响，从代谢角度探讨KXS升高单胺类神经递质水平的机制，采用体外酶反应体系和C6神经胶质细胞，观察不同浓度KXS对MAO-A和MAO-B活性的影响。在动物实验中，观察不同浓度KXS灌胃给药1、2、3周后对正常大鼠和慢性不可预测性温和应激（CMS）模型大鼠脑线粒体MAO-A和MAO-B活性的影响。结果显示，10 g•L⁻¹ KXS可显著降低酶反应体系中MAO-A和MAO-B的活性；在C6细胞中，0.625 - 10 g•L⁻¹浓度范围内的KXS对MAO-A活性无显著影响，但对MAO-B活性有明显的剂量依赖性抑制作用。KXS作用1、2、3周后对正常大鼠脑内MAO-A和MAO-B活性无显著影响。338 mg•kg⁻¹剂量KXS治疗2、3周后，CMS大鼠脑内MAO-A活性较模型组降低（P<0.05），而KXS治疗1、2、3周后对MAO-B活性无显著影响。结果表明，KXS对体外MAO-A和MAO-B活性有一定作用，对正常大鼠体内脑MAO-A和MAO-B活性无影响，对CMS大鼠脑内MAO-A活性有一定抑制作用。
