Gharagozloo F, Melendez F J, Hein R A, Laurence R G, Shemin R J, DiSesa V J, Cohn L H
Department of Surgery, Harvard Medical School, Boston, MA.
Circulation. 1987 Nov;76(5 Pt 2):V65-70.
The present study was undertaken to assess the effect of L-glutamate on the extended preservation of the heart ex vivo added to a hypothermic preservative solution. Ten sheep hearts were removed after application of cold crystalloid potassium cardioplegia. The hearts were immersed in iced normal saline and right ventricular and left ventricular intraventricular balloons were connected to transducers to measure compliance. Thermistor probes were placed in the interventricular septum. The heart was mounted on a retrograde perfusion apparatus via the cannula in the brachiocephalic trunk and placed in an isolated glass cylinder. The perfusion apparatus consisted of a roller pump, a heat exchanger, and a pediatric membrane oxygenator. Control determinations and those after 8 hr of preservation were made while the hearts were perfused with enriched autologous blood. Right and left ventricular compliance, left ventricular systolic and diastolic pressures, right ventricular systolic and diastolic pressures, left ventricular dP/dt, coronary arteriovenous oxygen consumption, lactate production, and heart weight were measured. Results showed significantly improved left and right ventricular systolic function with L-glutamate preservation. Whole heart oxygen consumption in the L-glutamate group showed a 4% increase, while the placebo group showed a significant decrease in oxygen consumption after 8 hr of preservation. The glutamate-preserved hearts also showed no change in lactate production after 8 hr of preservation as compared with a 81% increase in the placebo group. After 8 hr of perfusion there was 67% increase in the heart weight of the control and a 42% increase in the L-glutamate group.(ABSTRACT TRUNCATED AT 250 WORDS)
本研究旨在评估添加到低温保存液中的L-谷氨酸对离体心脏延长保存的效果。在应用冷晶体钾停搏液后，取出10个绵羊心脏。将心脏浸入冰生理盐水中，右心室和左心室内的球囊连接到传感器以测量顺应性。热敏电阻探头置于室间隔。心脏通过头臂干中的插管安装在逆行灌注装置上，并放置在一个单独的玻璃圆筒中。灌注装置由一个滚轴泵、一个热交换器和一个小儿膜式氧合器组成。在用富含自体血灌注心脏时，进行对照测定以及保存8小时后的测定。测量右心室和左心室顺应性、左心室收缩压和舒张压、右心室收缩压和舒张压、左心室dP/dt、冠状动脉动静脉氧耗、乳酸生成和心脏重量。结果显示，L-谷氨酸保存可显著改善左心室和右心室收缩功能。L-谷氨酸组全心脏氧耗增加4%，而安慰剂组在保存8小时后氧耗显著降低。与安慰剂组增加81%相比，L-谷氨酸保存的心脏在保存8小时后乳酸生成也没有变化。灌注8小时后，对照组心脏重量增加67%，L-谷氨酸组增加42%。（摘要截短至250字）
