The role of the pineal gland and melatonin in the regulation of the sexual processes of the male rat has been studied by means of the O2-uptake of brain areas that are involved in these processes, i.e. the hypothalamus, amygdala, septal area and posterior cortex. 2. Inhibition of the O2-uptake of the above mentioned nervous structures was observed in the blinded and/or melatonin-treated rats or castrated + blinded rats. 3. The testes also showed a decreased O2-uptake when the rats were blinded and/or melatonin-treated.