Rodríguez-Velázquez Eustolia, Taboada Pablo, Alatorre-Meda Manuel
Facultad de Odontología, Universidad Autónoma de Baja California, Campus Tijuana, Calzada Universidad 14418, 22390 Tijuana, B. C., Mexico; Departamento de Estomatología, Facultad de Odontología, Universidad de Santiago de Compostela, Campus Norte S/N, E-15782 Santiago de Compostela, Spain; Instituto de Ortopedia y Banco de Tejidos Musculoesqueléticos, Universidad de Santiago de Compostela, Campus Sur S/N, E-15782 Santiago de Compostela, Spain; Grupo de Física de Coloides y Polímeros, Departamento de Física de Materia Condensada, Facultad de Física, Universidad de Santiago de Compostela, Campus Sur S/N, E-15782 Santiago de Compostela, Spain.
Grupo de Física de Coloides y Polímeros, Departamento de Física de Materia Condensada, Facultad de Física, Universidad de Santiago de Compostela, Campus Sur S/N, E-15782 Santiago de Compostela, Spain.
Colloids Surf B Biointerfaces. 2017 Nov 1;159:898-904. doi: 10.1016/j.colsurfb.2017.08.055. Epub 2017 Aug 31.
Macroscopic hollow polymeric particles are attractive materials for various applications such as surgery, food industry, agriculture, etc. However, protocols reporting their synthesis have hitherto made use of organic solvents and/or sacrificial templates, compromising the encapsulation of different bioactive compounds and the process yield. Here, millimeter-size, hollow polymeric particles were synthesized, for the first time, in a solvent- and template free manner onto superhydrophobic surfaces (SHS). The particles were produced upon assembly and double superficial crosslinking of liquid droplets of DNA and methacrylamide chitosan aqueous solutions (CH:MA), leading to liquid-core particles with a hardened hydrogel shell. The particles displayed appealing physical and biological properties. The millimeter-size hydrogel shell, resulting from the double ionic/covalent crosslinking of CH:MA, endowed the hollow particles with softness to the touch and an outstanding structural stability against manipulation by hand and with forceps. Meanwhile, the liquid DNA core guaranteed a biocompatible cell encapsulation followed by a superior release and proliferation of viable cells, as compared to solid CH:MA particles prepared as a blank. Particles with these characteristics show promise for surgical protocols practiced in Tissue Engineering and Regenerative Medicine, where manipulable and biocompatible synthetic implants are often needed to supply living cells and other sensitive bioactive compounds.
宏观中空聚合物颗粒是用于手术、食品工业、农业等各种应用的有吸引力的材料。然而，迄今为止报道其合成的方案使用了有机溶剂和/或牺牲模板，这损害了不同生物活性化合物的包封和工艺产率。在此，首次以无溶剂和无模板的方式在超疏水表面（SHS）上合成了毫米尺寸的中空聚合物颗粒。这些颗粒是在DNA和甲基丙烯酰胺壳聚糖水溶液（CH:MA）的液滴组装和双重表面交联后产生的，形成了具有硬化水凝胶壳的液芯颗粒。这些颗粒表现出吸引人的物理和生物学特性。由CH:MA的双重离子/共价交联产生的毫米尺寸水凝胶壳，使中空颗粒触感柔软，并且具有出色的结构稳定性，能够抵抗手工和镊子操作。同时，与作为空白制备的固体CH:MA颗粒相比，液体DNA核心保证了生物相容性细胞包封，随后活细胞具有优异的释放和增殖能力。具有这些特性的颗粒在组织工程和再生医学中实施的手术方案中显示出前景，在这些领域中，通常需要可操作且生物相容的合成植入物来提供活细胞和其他敏感生物活性化合物。
