Botanical Gardens, Graduate School of Science, The University of Tokyo, Tokyo 112-0001, Japan.
Division of Biological Science, Graduate School of Science, Nagoya University, Nagoya 464-8602, Japan.
Plant Cell. 2017 Oct;29(10):2644-2660. doi: 10.1105/tpc.17.00255. Epub 2017 Sep 12.
Ribosome-related mutants in share several notable characteristics regarding growth and development, which implies the existence of a common pathway that responds to disorders in ribosome biogenesis. As a first step to explore this pathway genetically, we screened a mutagenized population of (), a temperature-sensitive mutant that is impaired in pre-rRNA processing, and isolated (), a suppressor mutant in which the defects of cell proliferation observed in at the restrictive temperature was markedly rescued. was identified as a missense mutation of the NAC transcription factor gene The mutation greatly alleviated the developmental abnormalities of and four other tested ribosome-related mutants, including However, the impaired pre-rRNA processing in and was not relieved by Expression of was localized to regions where phenotypic effects of ribosome-related mutations are readily evident and was elevated in and compared with the wild type. These findings suggest that ANAC082 acts downstream of perturbation of biogenesis of the ribosome and may mediate a set of stress responses leading to developmental alterations and cell proliferation defects.
核糖体相关突变体在共享生长和发育方面的几个显著特征，这意味着存在一个共同的途径来响应核糖体生物发生的障碍。作为从遗传学上探索这一途径的第一步，我们筛选了一个（）的诱变群体，这是一个温度敏感的突变体，在前 rRNA 加工中受损，并分离出（），这是一个抑制突变体，在该突变体中，在限制温度下观察到的细胞增殖缺陷在中明显得到挽救。被鉴定为 NAC 转录因子基因的错义突变 突变极大地减轻了和其他四个测试的核糖体相关突变体的发育异常，包括 然而，在 中，前 rRNA 加工的缺陷并没有被 缓解。在 中表达定位于易于观察到核糖体相关突变表型效应的区域，并与野生型相比，在 中升高。这些发现表明，ANAC082 作为核糖体生物发生障碍的下游作用，并可能介导一组应激反应，导致发育改变和细胞增殖缺陷。
