Niknami Zohreh, Eslamifar Ali, Emamirazavi Amirnader, Ebrahimi Alireza, Shirkoohi Reza
Department of Genetics, Faculty of Science, Islamic Azad University, Damghan, Iran.
Department of Clinical Research, Pasteur Institute of Iran, Tehran, Iran.
EXCLI J. 2017 Jul 10;16:1009-1017. doi: 10.17179/excli2017-481. eCollection 2017.
Colorectal cancer is the most common malignancy of the gastrointestinal tract with very high mortality. One of the most distinguishing features for the establishment of an epithelial-mesenchymal transition phenotype is the alteration of mesenchymal markers expression and structural adhesion proteins. We evaluated the significance of vimentin and fibronectin gene expression in relation to invasion and metastasis in CRC patients. Tissue specimens were collected consecutively from forty-five colorectal carcinoma patients during surgeries. Tissues were divided into two separate parts for pathological and molecular assays. In order to histological staging, tissue sections were prepared from formalin-fixed paraffin-embedded blocks and stained with Hematoxylin and Eosin. To quantify gene expression, specimens were dissected and homogenized. Moreover, SW480, SW48, SW948, Caco-2, HT-29 and LS174T as human colon cancer cell lines were obtained and cultured, then molecular analyzing was performed. As results the expression of gene increased in SW480, SW48 and SW948 while it decreased in Caco-2, HT-29 and LS174T. Moreover, was up-regulated in Caco-2, HT-29 and SW948, while it was down-regulated in SW480, SW48 and LS174T. In tissues, vimentin and fibronectin expression significantly increased in stromal cells, whereas vimentin decreased in colonic epithelial cells and fibronectin had no significant change. Vimentin and fibronectin expression were changed in tumor tissues. It was found an association between vimentin expression with age and tumor size; over-expression in older age and decreasing in larger tumor size. Furthermore, fibronectin over-expression is correlated to older age and high tumor stages; up-regulation with increasing age and high tumor stages.
结直肠癌是胃肠道最常见的恶性肿瘤，死亡率极高。上皮-间质转化表型确立的最显著特征之一是间充质标志物表达和结构黏附蛋白的改变。我们评估了波形蛋白和纤连蛋白基因表达在结直肠癌患者侵袭和转移方面的意义。在手术过程中连续收集了45例结直肠癌患者的组织标本。将组织分为两部分分别用于病理和分子检测。为了进行组织学分期，从福尔马林固定石蜡包埋块制备组织切片并用苏木精和伊红染色。为了定量基因表达，对标本进行解剖并匀浆。此外，获取并培养了SW480、SW48、SW948、Caco-2、HT-29和LS174T等人类结肠癌细胞系，然后进行分子分析。结果显示，该基因在SW480、SW48和SW948中表达增加，而在Caco-2、HT-29和LS174T中表达降低。此外，在Caco-2、HT-29和SW948中上调，而在SW480、SW48和LS174T中下调。在组织中，波形蛋白和纤连蛋白在基质细胞中的表达显著增加，而波形蛋白在结肠上皮细胞中减少，纤连蛋白无显著变化。波形蛋白和纤连蛋白在肿瘤组织中的表达发生改变。发现波形蛋白表达与年龄和肿瘤大小之间存在关联；在老年患者中过表达，在较大肿瘤中表达降低。此外，纤连蛋白过表达与老年和高肿瘤分期相关；随着年龄增长和肿瘤分期升高而上调。
