Faculty of Mathematics and Physics, Charles University, Ke Karlovu 3, 12116, Prague, Czech Republic.
Institute of Cellular and Molecular Botany, University of Bonn, Kirschallee 1, 53115, Bonn, Germany.
J Bioenerg Biomembr. 2017 Oct;49(5):391-397. doi: 10.1007/s10863-017-9724-x. Epub 2017 Sep 12.
According to the common view, weak acid uncouplers increase proton conductance of biological (and phospholipid bilayer) membranes, thus effecting H fluxes driven by their electrochemical gradients. Under certain conditions, however, uncouplers can induce unexpected effects opposite to the dissipation of H gradients. Results are presented here demonstrating CCCP-induced proton influx into Saccharomyces cerevisiae cytosol driven by the electrochemical potentials of CCCP and its CCCP anions, independent of electrochemical H-gradient. Another view of week acid uncouplers' action is proposed that is logically consistent with these observations.
根据普遍观点，弱酸解偶联剂会增加生物（和磷脂双层）膜的质子传导率，从而影响由其电化学梯度驱动的 H 流。然而，在某些条件下，解偶联剂会产生与 H 梯度耗散相反的意外效果。本文介绍了 CCCP 诱导的质子内流进入酿酒酵母细胞质的结果，该过程由 CCCP 及其 CCCP 阴离子的电化学势驱动，而与电化学 H 梯度无关。提出了另一种弱酸解偶联剂作用的观点，该观点与这些观察结果在逻辑上是一致的。
