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多金属氧酸盐作为唾液酸酶模拟物：磷酸钨促进从糖蛋白中选择性且无损地去除唾液酸

Polyoxometalates as sialidase mimics: selective and non-destructive removal of sialic acid from a glycoprotein promoted by phosphotungstic acid.

作者信息

Van Rompuy Laura Sofia, Parac-Vogt Tatjana N

机构信息

Department of Chemistry, KU Leuven, Celestijnenlaan 200F, 3001, Leuven, Belgium.

出版信息

Chem Commun (Camb). 2017 Sep 21;53(76):10600-10603. doi: 10.1039/c7cc05888h.

DOI:10.1039/c7cc05888h
PMID:28902191
Abstract

The selective hydrolysis of the glycosidic bond between the terminal sialic acid and the penultimate sugar has been achieved in the alpha-2-HS-glycoprotein (Fetuin-A) in the presence of HPWO, a Keggin type polyoxometalate. A controlled liberation of sialic acid from the glycoprotein could be achieved at pH 3.0 and 37 °C without the destruction of the protein backbone.

摘要

在Keggin型多金属氧酸盐HPWO存在的情况下，已实现了α-2-HS-糖蛋白（胎球蛋白-A）中末端唾液酸与倒数第二个糖之间糖苷键的选择性水解。在pH 3.0和37°C条件下，可以从糖蛋白中可控地释放唾液酸，而不会破坏蛋白质主链。

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