基于第三外显子中的一个多态性限制性位点检测到HLA - DZα的两个等位基因。

Two alleles detected for HLA-DZ alpha on the basis of a polymorphic restriction site in the third exon.

作者信息

Bosch M L, Slierendregt B L, Tilanus M G, Giphart M J

机构信息

Department of Immunohaematology and Blood Bank, University Hospital, Leiden, The Netherlands.

出版信息

Tissue Antigens. 1987 Aug;30(2):84-90. doi: 10.1111/j.1399-0039.1987.tb01602.x.

DOI:10.1111/j.1399-0039.1987.tb01602.x

PMID:2890222

Abstract

The restriction enzyme ApaI has been used to define Restriction Fragment Length Polymorphism (RFLP) within the DZ alpha gene. Digestion of genomic DNA of 96 individuals with ApaI reveals the existence of two different patterns. Both homozygous and heterozygous individuals were detected. Hardy-Weinberg analysis indicates that DZ alpha is part of a di-allelic system with allele frequencies of 50%. DZ alpha polymorphism in the population studied does not correlate with serologically determined class I and HLA-DR, DQ and cellular DP typing data. A limited family study demonstrates that DZ alpha polymorphism segregates with HLA-class II phenotypes.

摘要

限制性内切酶ApaI已被用于确定DZα基因内的限制性片段长度多态性（RFLP）。用ApaI对96个个体的基因组DNA进行消化，发现存在两种不同的模式。同时检测到了纯合子和杂合子个体。哈迪-温伯格分析表明，DZα是一个双等位基因系统的一部分，等位基因频率为50%。在所研究的人群中，DZα多态性与血清学确定的I类以及HLA-DR、DQ和细胞DP分型数据不相关。一项有限的家系研究表明，DZα多态性与HLA-II类表型共分离。