Galindo Gizela Melina, Rodrigues Robson Andrade, Marcondes Sandriely Fernanda, Soares Priscilla, Tavares Luiz Eduardo Roland, Fernandes Carlos Eurico
Laboratório de Patologia Experimental, Centro de Ciências Biológicas e da Saúde - CCBS, Universidade Federal de Mato Grosso do Sul - UFMS, Campo Grande, MS, Brasil.
Laboratório de Parasitologia Animal, Centro de Ciências Biológicas e da Saúde - CCBS, Universidade Federal de Mato Grosso do Sul - UFMS, Campo Grande, MS, Brasil.
Rev Bras Parasitol Vet. 2017 Jul-Sep;26(3):285-291. doi: 10.1590/S1984-29612017044. Epub 2017 Aug 24.
The aim of this study was to determine the morphometric measures and morphological aspects of nematode-cysts in Gymnotus inaequilabiatus, and the presence of melanomacrophage centers (MMCs) associated with the periphery of cysts and in the liver parenchyma. Adult specimens, 34 female (123.1 ± 43.9g) and 45 male (135.5 ± 43.4g), from Paraguay River, Corumbá, Brazil, were used. The number of nematode-cysts was determined in 79 livers and 25 of them randomly selected for histopathological analysis and morphometric measures of nematode-cysts (mean diameter, thickness of collagen layer, and cyst-wall layer). The percentage of cysts with MMCs on the periphery and density in the liver parenchyma was estimated. The average number of macroscopic cysts was of 48.7 ± 2.78. Granulomatous reaction was observed surrounding the cysts. Diameter, collagen layer and cyst-wall measurements were 293.0 ± 75.18 (µm), 17.72 ± 6.01 (µm) and 12.21 ± 9.51 (µm), respectively. The number of nematode-cysts was correlated with hepatosomatic index, (r=0.26, P<0.05). Collagen layer was correlated with cyst diameter (r=0.62, P<0.01). Pericystic and parenchymatous MMCs were moderately (r=0.48) and highly (r=0.90) correlated with nematode-cysts number. Morphological characteristics of hepatic tissue and cysts-nematodes measures suggest that G. inaequilabiatus acts as a paratenic host to nematodes in the larval stage.
本研究的目的是确定不等长电鳗（Gymnotus inaequilabiatus）中线虫囊肿的形态测量指标和形态学特征，以及与囊肿周边和肝实质相关的黑色素巨噬细胞中心（MMCs）的存在情况。使用了来自巴西科伦巴巴拉圭河的成年标本，其中34只雌性（123.1±43.9克）和45只雄性（135.5±43.4克）。在79个肝脏中确定了线虫囊肿的数量，并随机选择其中25个进行组织病理学分析和线虫囊肿的形态测量（平均直径、胶原层厚度和囊肿壁层）。估计了周边有MMCs的囊肿百分比和肝实质中的密度。宏观囊肿的平均数量为48.7±2.78。在囊肿周围观察到肉芽肿反应。直径、胶原层和囊肿壁的测量值分别为293.0±75.18（μm）、17.72±6.01（μm）和12.21±9.51（μm）。线虫囊肿的数量与肝体指数相关（r=0.26，P<0.05）。胶原层与囊肿直径相关（r=0.62，P<0.01）。囊肿周围和实质中的MMCs与线虫囊肿数量呈中度（r=0.48）和高度（r=0.90）相关。肝组织的形态学特征和囊肿-线虫测量表明，不等长电鳗在幼虫阶段作为线虫的转续宿主。
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