Rodrigues Robson A, Silva Eduarda S, Marcondes Sandriely F, Galindo Gizela M, Oliveira Gustavo G DE, Souza Alda I DE, Ragusa-Netto José, Fernandes Carlos E
Programa de Pós-Graduação em Ciência Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade Federal de Mato Grosso do Sul, Av. Senador Filinto Müller, 2443, 79070-900 Campo Grande, MS, Brazil.
Laboratório de Patologia Experimental, Instituto de Biociências, Universidade Federal de Mato Grosso do Sul, Av. Costa e Silva, s/n, Bairro Universitário, 79070-900 Campo Grande, MS, Brazil.
An Acad Bras Cienc. 2018 Jan-Mar;90(1):49-57. doi: 10.1590/0001-3765201720150824. Epub 2018 Feb 1.
This study describes the hematological and biometric characteristics of male and female Gymnotus species from the Pantanal, Mato Grosso do Sul state, Brazil. Fifty adult specimens of Gymnotus inaequilabiatus were weighed, measured, and then euthanized. Blood was collected by puncturing the celiac mesenteric vein to determine the hematocrit, hemoglobin content, number of erythrocytes, mean corpuscular volume, mean corpuscular hemoglobin concentration, glucose level, absolute value of leukocytes, and relative value of leukocytes and thrombocytes. Body weight and relative condition factor did not differ (P > 0.05) between the sexes, as well as erythrogram and the blood glucose values. Hematocrit ranged from 18.0% to 54.0%; hemoglobin from 1.1 to 14.7 g dL-1; number of erythrocytes from 0.2 × 106 to 3.8 ×106 µL-1; MCV from 24.2 to 321.7 fL; and MCHC from 4.2 to 44.5 g dL-1. In the differential count were identified thrombocytes, lymphocytes, neutrophils, monocytes, basophils, immature leukocytes, and PAS-positive granular leukocyte (PAS-GL). Females had a higher percentage of immature leukocytes (P < 0.05) than males. Glucose levels, erythrogram, leukogram, and the morphology of defense cells are comparable to other fish species of the Pantanal. Thrombocytes were the most frequent defense cells, followed by lymphocytes and neutrophils.
本研究描述了来自巴西南马托格罗索州潘塔纳尔湿地的裸背电鳗属雌雄物种的血液学和生物特征。对50只成年不等侧裸背电鳗标本进行称重、测量，然后实施安乐死。通过穿刺腹腔肠系膜静脉采集血液，以测定血细胞比容、血红蛋白含量、红细胞数量、平均红细胞体积、平均红细胞血红蛋白浓度、血糖水平、白细胞绝对值以及白细胞和血小板的相对值。体重和相对状况因子在两性之间没有差异（P>0.05），红细胞图和血糖值也是如此。血细胞比容范围为18.0%至54.0%；血红蛋白为1.1至14.7 g dL-1；红细胞数量为0.2×106至3.8×106 µL-1；平均红细胞体积为24.2至321.7 fL；平均红细胞血红蛋白浓度为4.2至44.5 g dL-1。在分类计数中识别出了血小板、淋巴细胞、中性粒细胞、单核细胞、嗜碱性粒细胞、未成熟白细胞和PAS阳性颗粒白细胞（PAS-GL）。雌性未成熟白细胞的百分比高于雄性（P<0.05）。血糖水平、红细胞图、白细胞图以及防御细胞的形态与潘塔纳尔湿地的其他鱼类物种相当。血小板是最常见的防御细胞，其次是淋巴细胞和中性粒细胞。
