坐骨神经修复中与富血小板血浆（PRP）相关的翻转式和标准静脉移植物。一项组织形态计量学研究。

Inside-out and standard vein grafts associated with platelet-rich plasma (PRP) in sciatic nerve repair. A histomorphometric study.

作者信息

Roque José Sidney, Pomini Karina Torres, Buchaim Rogério Leone, Buchaim Daniela Vieira, Andreo Jesus Carlos, Roque Domingos Donizeti, Rodrigues Antonio de Castro, Rosa Geraldo Marco, Moraes Luis Henrique Rapucci, Viterbo Fausto

机构信息

PhD, Department of Anatomy, Universidade Estadual do Norte do Paraná (UENP), Jacarezinho-PR, Brazil. Acquisition, analysis and interpretation of data; technical procedures.

MSc, Department of Biological Sciences, Faculdade de Odontologia de Bauru, Universidade de São Paulo (FOB-USP), Bauru-SP, Brazil. Manuscript preparation and writing.

出版信息

Acta Cir Bras. 2017 Aug;32(8):617-625. doi: 10.1590/s0102-865020170080000003.

DOI:10.1590/s0102-865020170080000003

PMID:28902937

Abstract

PURPOSE

: To evaluated the tubulization technique with standard and inside-out vein, filled or not with platelet-rich plasma (PRP), in sciatic nerve repair.

METHODS

: Seventy male Wistar rats were randomly divided into five groups: IOVNF (Inside-Out Vein with No Filling); IOVPRP (Inside-Out Vein filled with PRP); SVNF (Standard Vein with No Filling); SVPRP (Standard Vein filled with PRP); Sham (Control). The left external jugular vein was used as graft in a 10 mm nervous gap.

RESULTS

: In the morphological analysis of all groups, myelinated nerve fibers with evident myelin sheath, neoformation of the epineurium and perineurium, organization of intraneural fascicles and blood vessels were observed. In the morphometry of the distal stump fibers, SVPRP group had the highest means regarding fiber diameter (3.63±0.42 μm), axon diameter (2.37±0.31 μm) and myelin sheath area (11.70±0.84 μm2). IOVPRP group had the highest means regarding axon area (4.39±1.16 μm2) and myelin sheath thickness (0.80±0.19 μm). As for values of the fiber area, IOVNF group shows highest means (15.54±0.67 μm2), but are still lower than the values of the Sham group.

CONCLUSION

: The graft filled with platelet-rich plasma, with use standard (SVPRP) or inside-out vein (IOVPRP), promoted the improvement in axonal regeneration on sciatic nerve injury.

摘要

目的

评估在坐骨神经修复中使用标准静脉和翻转静脉、填充或不填充富血小板血浆（PRP）的管状化技术。

方法

70只雄性Wistar大鼠随机分为五组：IOVNF（未填充的翻转静脉）；IOVPRP（填充PRP的翻转静脉）；SVNF（未填充的标准静脉）；SVPRP（填充PRP的标准静脉）；假手术组（对照组）。将左颈外静脉用作10毫米神经缺损的移植物。

结果

在所有组的形态学分析中，观察到有明显髓鞘的有髓神经纤维、神经外膜和神经束膜的新形成、神经内束和血管的组织。在远端残端纤维的形态测量中，SVPRP组在纤维直径（3.63±0.42μm）、轴突直径（2.37±0.31μm）和髓鞘面积（11.70±0.84μm²）方面的平均值最高。IOVPRP组在轴突面积（4.39±1.16μm²）和髓鞘厚度（0.80±0.19μm）方面的平均值最高。至于纤维面积的值，IOVNF组的平均值最高（15.54±0.67μm²），但仍低于假手术组的值。