Department of Pathogenic Microbiology and Immunology, School of Basic Medical Sciences, Xi'an Jiaotong University, Xi'an, China.
Department of Pharmaceutics, School of Pharmacy, China Pharmaceutical University, Nanjing, China.
Biomed Pharmacother. 2017 Nov;95:865-874. doi: 10.1016/j.biopha.2017.08.101. Epub 2017 Sep 10.
The gut microbiota has a crucial effect on human health and physiology. Hypothyroid Hashimoto's thyroiditis (HT) is an autoimmune disorder manifested with environmental and genetic factors. However, it is hypothesized that intestinal microbes might play a vital role in the pathogenesis of HT. The aim of current was to investigate and characterize the gut microbial composition of HT patients both quantitatively and qualitatively. The fecal samples from 29 HT patients and 12 healthy individuals were collected. The PCR-DGGE targeted V3 site of 16S rRNA gene and real time PCR for Bifidobacterium Lactobacillus, Bacteroides vulgatus and Clostridium leptum were performed. Pyrosequencing of 16S rRNA gene with V4 location was performed on 20 randomly selected samples. The comparative analysis of diversity and richness indices revealed diversification of gut microbiota in HT as compared to control. The statistical data elucidate the alterations in phyla of HT patients which was also affirmed at the family level. We observed the declined abundance of Prevotella_9 and Dialister, while elevated genera of the diseased group included Escherichia-Shigella and Parasutterella. The alteration in gut microbial configuration was also monitored at the species level, which showed an increased abundance of E. coli in HT. Therefore, the current study is in agreement with the hypothesis that HT patients have intestinal microbial dysbiosis. The taxa statistics at species-level along with each gut microbial community were modified in HT. Thus, the current study may offer the new insights into the treatment of HT patients, disease pathway, and mechanism.
肠道微生物群对人类健康和生理有至关重要的影响。桥本氏甲状腺功能减退症（HT）是一种自身免疫性疾病，表现出环境和遗传因素。然而，据推测肠道微生物可能在 HT 的发病机制中发挥重要作用。本研究旨在定量和定性地研究 HT 患者的肠道微生物组成。收集了 29 名 HT 患者和 12 名健康个体的粪便样本。使用 PCR-DGGE 靶向 16S rRNA 基因的 V3 位点和实时 PCR 检测双歧杆菌、乳酸杆菌、脆弱拟杆菌和梭状芽孢杆菌。对 20 个随机选择的样本进行 16S rRNA 基因 V4 位置的焦磷酸测序。多样性和丰富度指数的比较分析表明，与对照组相比，HT 患者的肠道微生物群多样化。统计数据阐明了 HT 患者门水平的变化，这在科水平也得到了证实。我们观察到普雷沃氏菌属 9 和 Dialister 的丰度降低，而患病组的属包括 Escherichia-Shigella 和 Parasutterella 的丰度增加。在物种水平上也监测到肠道微生物结构的改变，HT 中大肠杆菌的丰度增加。因此，本研究与 HT 患者存在肠道微生物失调的假设一致。HT 患者的物种水平分类统计数据以及每个肠道微生物群落都发生了改变。因此，本研究可能为 HT 患者的治疗、疾病途径和机制提供新的见解。
