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肠道微生物失调及其与食管癌的关系。

Gut microbial dysbiosis and its association with esophageal cancer.

机构信息

University of Veterinary and Animal Sciences, Department of Clinical Sciences, Section of Epidemiology and public Health, Lahore Sub-campus Jhang, Pakistan.

Muhammad Nawaz Shareef University of Agriculture Multan, Department of Veterinary and Animal Sciences, Multan, Pakistan.

出版信息

J Appl Biomed. 2021 Mar;19(1):1-13. doi: 10.32725/jab.2021.005. Epub 2021 Feb 3.

Abstract

Due to its aggressive nature and low survival rate, esophageal cancer is one of the deadliest cancer. While the intestinal microbiome significantly influences human health and disease. This research aimed to investigate and characterize the relative abundance of intestinal bacterial composition in esophageal cancer patients. The fecal samples were collected from esophageal cancer patients (n = 15) and healthy volunteers (n = 10). The PCR-DGGE was carried out by focusing on the V3 region of the 16S rRNA gene, and qPCR was performed for Bacteroides vulgatus, Escherichia coli, Bifidobacterium, Clostridium leptum and Lactobacillus. High-throughput sequencing of the 16S rRNA gene targeting the V3+V4 region was performed on 20 randomly selected samples. PCR-DGGE and High-throughput diversity results showed a significant alteration of gut bacterial composition between the experimental and control groups, which indicates the gut microbial dysbiosis in esophageal cancer patients. At the phylum level, there was significant enrichment of Bacteroidetes, while a non-significant decrease of Firmicutes in the experimental group. At family statistics, a significantly higher level of Bacteroidaceae and Enterobacteriaceae, while a significantly lower abundance of Prevotellaceae and Veillonellaceae were observed. There was a significantly high prevalence of genera Bacteroides, Escherichia-Shigella, while a significantly lower abundance of Prevotella_9 and Dialister in the experimental group as compared to the control group. Furthermore, the species analysis also showed significantly raised level of Bacteroides vulgatus and Escherichia coli in the experimental group. These findings revealed a significant gut microbial dysbiosis in esophageal cancer patients. So, the current study can be used for the understanding of esophageal cancer treatment, disease pathway, mechanism, and probiotic development.

摘要

由于其侵袭性和低生存率,食管癌是最致命的癌症之一。而肠道微生物群显著影响人类健康和疾病。本研究旨在调查和描述食管癌患者肠道细菌组成的相对丰度。采集食管癌患者(n=15)和健康志愿者(n=10)的粪便样本。通过聚焦 16S rRNA 基因的 V3 区进行 PCR-DGGE,并用 qPCR 检测脆弱拟杆菌、大肠杆菌、双歧杆菌、梭状芽孢杆菌和乳酸杆菌。对 20 个随机选择的样本进行 16S rRNA 基因 V3+V4 区的高通量测序。PCR-DGGE 和高通量多样性结果表明,实验组和对照组之间肠道细菌组成有显著改变,表明食管癌患者肠道微生物失调。在门水平上,实验组中拟杆菌门显著富集,厚壁菌门显著减少。在科水平的统计中,拟杆菌科和肠杆菌科的水平显著升高,而普雷沃氏菌科和韦荣球菌科的丰度显著降低。与对照组相比,实验组中拟杆菌属、大肠埃希氏菌-志贺氏菌属的丰度显著升高,而普雷沃氏菌_9 属和戴阿利斯特菌属的丰度显著降低。此外,种水平的分析也显示实验组中脆弱拟杆菌和大肠杆菌的水平显著升高。这些发现揭示了食管癌患者肠道微生物失调的显著特征。因此,本研究可以用于理解食管癌的治疗、疾病途径、机制和益生菌的开发。

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