Ramos Daniel, Calleja Montserrat, Mertens Johann, Zaballos A, Tamayo Javier
BioNanoMechanics Lab, National Centre for Microelectronics, IMM-CNM, CSIC, Isaac Newton 8 (PTM), Tres Cantos 28760, Madrid, Spain.
Genomics Functional Unit, Department of Immunology and Oncology, CNB-CSIC, Darwin 3, Madrid 28049, Spain.
Sensors (Basel). 2007 Sep 7;7(9):1834-1845. doi: 10.3390/s7091834.
When microcantilevers are used in the dynamic mode, the resonance shift uponmaterial adsorption depends on the position of the adsorbate along the microcantilever. Wehave previously described that the adsorbate stiffness needs to be considered in addition toits mass in order to correctly interpret the resonance shift. Here we describe a method thatallows obtaining the Young's modulus of the adsorbed bacteria derived from themeasurement of the frequency shift when adsorbates are placed close to the clampingregion. As a model system we have used E. Coli bacteria deposited on the cantileversurface by the ink-jet technique. We demonstrate that the correct information aboutadsorbed mass can be extracted by recording the cantilever profile and its resonanceresponse. Also, the position and extent of adsorbates is determined by recording themicrocantilever profile. We use a theoretical model based on the Euler - Bernouilliequation for a beam with both mass and flexural rigidity local increase due to the depositedmaterial.
当微悬臂梁用于动态模式时，材料吸附引起的共振频率偏移取决于吸附质沿微悬臂梁的位置。我们之前曾描述过，除了吸附质的质量外，还需要考虑其刚度，以便正确解释共振频率偏移。在此，我们描述一种方法，该方法通过测量吸附质靠近固定区域时的频率偏移来获取吸附细菌的杨氏模量。作为模型系统，我们使用通过喷墨技术沉积在悬臂梁表面的大肠杆菌。我们证明，通过记录悬臂梁轮廓及其共振响应，可以提取有关吸附质量的正确信息。此外，通过记录微悬臂梁轮廓来确定吸附质的位置和范围。我们使用基于欧拉 - 伯努利方程的理论模型，该模型适用于由于沉积材料导致质量和抗弯刚度局部增加的梁。
