Adam Vojtech, Mikelova Radka, Hubalek Jaromír, Hanustiak Pavel, Beklova Miroslava, Hodek Petr, Horna Ales, Trnkova Libuse, Stiborova Marie, Zeman Ladislav, Kizek Rene
Department of Chemistry and Biochemistry, Mendel University of Agriculture and Forestry, Zemedelska 1, CZ-613 00 Brno, Czech Republic.
Department of Chemistry, Faculty of Science, Masaryk University, Kotlarska 37, CZ-611 37 Brno, Czech Republic.
Sensors (Basel). 2007 Oct 19;7(10):2402-2418. doi: 10.3390/s7102402.
About biological affecting of flavonoids on animal organisms is known less,thus we selected flavonoids, flavanones and flavones, and their glycosides, which wereexamined as potential inducers of cytochrome(s) P450 when administrated by gavages intoexperimental male rats. The study was focused on induction of CYP1A1, the majorcytochrome P450 involved in carcinogen activation. The data obtained demonstrate thenecessity of taking into account not only ability of flavonoids to bind to Ah receptor(induction factor) but also to concentrate on their distribution and metabolism (includingcolon microflora) in the body. After that we examined certain flavonoids as potential inducers of cytochrome P450, we wanted to suggest and optimize suitable electrochemical technique for determination of selected flavonoids (quercetin, quercitrin, rutin, chrysin and diosmin) in body liquids. For these purposes, we selected square wave voltannetry using carbon paste electrode. Primarily we aimed on investigation of their basic electrochemical behaviour. After that we have optimized frequency, step potential and supporting electrolyte. Based on the results obtained, we selected the most suitable conditions for determination of the flavonoids as follows: frequency 180 Hz, step potential 1.95 mV/s and phosphate buffer of pH 7 as supporting electrolyte. Detection limits (3 S/N) of the flavonoids were from units to tens of nM except diosmin, where the limit were higher than μM. In addition, we attempted to suggest a sensor for analysis of flavonoids in urine. It clearly follows from the results obtained that flavonoids can be analysed in the presence of animal urine, because urine did not influence much the signals of flavonoids (recoveries of the signals were about 90 %).
关于黄酮类化合物对动物机体的生物学影响，人们了解得较少。因此，我们选择了黄酮类、黄烷酮类和黄酮类及其糖苷，通过灌胃给予实验雄性大鼠，研究它们作为细胞色素P450潜在诱导剂的情况。该研究聚焦于CYP1A1的诱导，CYP1A1是参与致癌物激活的主要细胞色素P450。所获得的数据表明，不仅要考虑黄酮类化合物与Ah受体（诱导因子）结合的能力，还要关注它们在体内的分布和代谢（包括结肠微生物群）。在研究了某些黄酮类化合物作为细胞色素P450的潜在诱导剂之后，我们想提出并优化一种合适的电化学技术，用于测定体液中选定的黄酮类化合物（槲皮素、槲皮苷、芦丁、白杨素和地奥司明）。为此，我们选择了使用碳糊电极的方波伏安法。首先，我们旨在研究它们的基本电化学行为。之后，我们优化了频率、步长电位和支持电解质。根据获得的结果，我们选择了测定黄酮类化合物的最合适条件如下：频率180Hz，步长电位1.95mV/s，pH7的磷酸盐缓冲液作为支持电解质。除地奥司明外，黄酮类化合物的检测限（3S/N）为单位至数十纳摩尔，地奥司明的检测限高于微摩尔。此外，我们试图提出一种用于分析尿液中黄酮类化合物的传感器。从获得的结果可以明显看出，黄酮类化合物可以在动物尿液存在的情况下进行分析，因为尿液对黄酮类化合物的信号影响不大（信号回收率约为90%）。
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