非羟基化黄酮类化合物作为大鼠肝脏细胞色素P450 1A和2B同工酶诱导剂的差异效应。

Differential effects of nonhydroxylated flavonoids as inducers of cytochrome P450 1A and 2B isozymes in rat liver.

作者信息

Canivenc-Lavier M C, Bentejac M, Miller M L, Leclerc J, Siess M H, Latruffe N, Suschetet M

机构信息

Unité de Toxicologie Nutritionnelle, Station de Recherche sur la Qualité des Aliments de l'Homme, INRA, Dijon, France.

出版信息

Toxicol Appl Pharmacol. 1996 Feb;136(2):348-53. doi: 10.1006/taap.1996.0042.

DOI:10.1006/taap.1996.0042

PMID:8619243

Abstract

Flavanone, flavone, and tangeretin differentially affected the activities of cytochrome P540 1A and 2B isozymes in rat liver. Flavone and, to a lesser extent, tangeretin, increased activities of ethoxyresorufin O-deethylase, methoxyresorufin O-demethylase, and pentoxyresorufin O-dealkylase (PROD), whereas flavanone mainly enhanced PROD activity. Immunoblot analysis indicated that flavone and tangeretin increased cytochrome P450 1A1, 1A2, and 2B1,2 forms, whereas flavanone only enhanced the cytochrome P450 2B isozymes. Northern blot study showed that flavone and tangeretin increased the level of the cytochrome P450 1A2 mRNAs. The concentration of the other mRNAs were slightly or not affected by flavonoids. These results suggest that the induction of P450 1A2 by flavone and tangeretin might involve a transcriptional and/or post-transcriptional mechanism.

摘要

黄烷酮、黄酮和橘皮素对大鼠肝脏中细胞色素P450 1A和2B同工酶的活性有不同影响。黄酮以及在较小程度上的橘皮素，可提高乙氧基试卤灵O - 脱乙基酶、甲氧基试卤灵O - 脱甲基酶和戊氧基试卤灵O - 脱烷基酶（PROD）的活性，而黄烷酮主要增强PROD活性。免疫印迹分析表明，黄酮和橘皮素可增加细胞色素P450 1A1、1A2以及2B1、2的形式，而黄烷酮仅增强细胞色素P450 2B同工酶。Northern印迹研究显示，黄酮和橘皮素可提高细胞色素P450 1A2 mRNA的水平。其他mRNA的浓度受黄酮类化合物的影响较小或未受影响。这些结果表明，黄酮和橘皮素对P450 1A2的诱导可能涉及转录和/或转录后机制。