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心肌缺血大鼠循环微泡对心肌缺血/再灌注损伤中心肌细胞凋亡的保护作用

Protective effects of circulating microvesicles derived from myocardial ischemic rats on apoptosis of cardiomyocytes in myocardial ischemia/reperfusion injury.

作者信息

Wang Yao, Wei Su, Wang Yi-Lu, Liu Miao, Shang Man, Zhang Qi, Wu Yan-Na, Liu Ming-Lin, Song Jun-Qiu, Liu Yan-Xia

机构信息

Department of Pharmacology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070, China.

Department of Dermatology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.

出版信息

Oncotarget. 2017 Apr 26;8(33):54572-54582. doi: 10.18632/oncotarget.17424. eCollection 2017 Aug 15.

DOI:10.18632/oncotarget.17424
PMID:28903365
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5589604/
Abstract

OBJECTIVE

To investigate the effects of circulating microvesicles derived from myocardial ischemia (I-MVs) on apoptosis in myocardial ischemia/reperfusion (I/R) injury in rats.

METHODS

I-MVs from rats undergoing myocardial left anterior descending (LAD) coronary artery ligation were isolated by ultracentrifugation from circulating blood and characterized by flow cytometry. I-MVs were administered intravenously (4.8 mg/kg) at 5 min before reperfusion procedure in I/R injury model which was induced by 30-min of ischemia and 120-min of reperfusion of LAD in rats.

RESULTS

Treatment with I-MVssignificantly reduced the size of myocardial infarction, the activities of serum CK-MB and LDH, and the number of apoptotic cardiomyocytes. The activities of caspase 3, caspase 9 and caspase 12 in myocardium were also decreased significantly with I-MVs treatment. Moreover, the expression of Bax was decreased but Bcl-2 was increased. The expression of glucose regulated protein 78 (GRP78), sarco/endoplasmic reticulum Ca-ATPase 2 (SERCA2) and phosphorylated phospholamban (p-PLB) were increased after being treated with I-MVs.

CONCLUSION

I-MVs could protect hearts from I/R injury in rats through SERCA2 and p-PLB of calcium regulatory proteins to alleviate intrinsic myocardial apoptosis including mitochondrial and endoplasmic reticulum pathways.

摘要

目的

探讨心肌缺血来源的循环微泡（I-MVs）对大鼠心肌缺血/再灌注（I/R）损伤中细胞凋亡的影响。

方法

通过超速离心从经历左前降支（LAD）冠状动脉结扎的大鼠循环血液中分离I-MVs，并通过流式细胞术进行鉴定。在大鼠LAD缺血30分钟和再灌注120分钟诱导的I/R损伤模型中，在再灌注程序前5分钟静脉注射I-MVs（4.8毫克/千克）。

结果

I-MVs治疗显著减小了心肌梗死面积、降低了血清肌酸激酶同工酶（CK-MB）和乳酸脱氢酶（LDH）活性以及凋亡心肌细胞数量。I-MVs治疗还显著降低了心肌中半胱天冬酶3、半胱天冬酶9和半胱天冬酶12的活性。此外，Bax表达降低而Bcl-2表达增加。用I-MVs处理后，葡萄糖调节蛋白78（GRP78）、肌浆网/内质网钙ATP酶2（SERCA2）和磷酸化受磷蛋白（p-PLB）的表达增加。

结论

I-MVs可通过钙调节蛋白SERCA2和p-PLB保护大鼠心脏免受I/R损伤，减轻包括线粒体和内质网途径在内的心肌细胞内凋亡。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fcbc/5589604/3123da915e49/oncotarget-08-54572-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fcbc/5589604/10c78e3c87e2/oncotarget-08-54572-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fcbc/5589604/dee1def80a5c/oncotarget-08-54572-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fcbc/5589604/411e55386e80/oncotarget-08-54572-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fcbc/5589604/5bf65de73635/oncotarget-08-54572-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fcbc/5589604/3123da915e49/oncotarget-08-54572-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fcbc/5589604/10c78e3c87e2/oncotarget-08-54572-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fcbc/5589604/dee1def80a5c/oncotarget-08-54572-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fcbc/5589604/411e55386e80/oncotarget-08-54572-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fcbc/5589604/5bf65de73635/oncotarget-08-54572-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fcbc/5589604/3123da915e49/oncotarget-08-54572-g005.jpg

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2
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Cardiology. 2016;134(2):109-17. doi: 10.1159/000443680. Epub 2016 Mar 4.
3
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Evid Based Complement Alternat Med. 2018 Apr 16;2018:3207973. doi: 10.1155/2018/3207973. eCollection 2018.
5
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