Kim Il-Hun, Park Jaejin, Suk Ho Young, Bae Han-Gyu, Min Mi-Sook, Tsai Tein-Shun, Park Daesik
a Department of Biology , Kangwon National University , Chuncheon , Kangwon-do , South Korea.
b Department of Life Sciences , Yeungnam University , Gyeongsan , Gyeongsanbuk-do , South Korea.
Mitochondrial DNA A DNA Mapp Seq Anal. 2018 Jul;29(5):772-777. doi: 10.1080/24701394.2017.1357710. Epub 2017 Sep 13.
To investigate the phylogenetic relationships of the genus Laticauda to related higher taxa, we compared the sequences of four mitochondrial genes (12S rRNA, 16S rRNA, ND4, Cytb) from three Laticauda species (L. colubrina, L. laticaudata, and L. semifasciata) with those of 55 Asian and Australo-Melanesian elapid species. We also characterized the complete mitogenomes of the three Laticauda species and compared the sequences of 13 mitochondrial genes from Laticauda species with five terrestrial elapid and one viperid species to estimate phylogenetic relationships and divergence times. Our results showed that the genus Laticauda is paraphyletic to terrestrial elapids and diverged from the Asian elapids approximately 16.23 Mya. The mitogenomes of the three Laticauda species commonly encoded 13 proteins, 22 tRNAs, 12S and 16S rRNAs and two control regions and ranged from 17,170 and 17,450 bp in size. The L. colubrina mitogenome was more similar to that of L. laticaudata than that of L. semifasciata. The divergence time among the three Laticauda clades was estimated at 8-10 Mya, and a close phylogenetic relationship between L. colubrina and L. laticaudata was found. Our results contribute to our understanding of the evolutionary history of sea kraits.
为了研究扁尾海蛇属与相关高级分类群的系统发育关系，我们比较了三种扁尾海蛇（蓝灰扁尾海蛇、扁尾海蛇、半环扁尾海蛇）的四个线粒体基因（12S rRNA、16S rRNA、ND4、Cytb）序列与55种亚洲和澳大拉西亚-美拉尼西亚眼镜蛇科蛇类的序列。我们还对这三种扁尾海蛇的完整线粒体基因组进行了特征分析，并将扁尾海蛇属的13个线粒体基因序列与五种陆生眼镜蛇科蛇类和一种蝰蛇科蛇类进行比较，以估计系统发育关系和分歧时间。我们的结果表明，扁尾海蛇属相对于陆生眼镜蛇科蛇类是并系的，并且大约在1623万年前从亚洲眼镜蛇科蛇类分化出来。这三种扁尾海蛇的线粒体基因组通常编码13种蛋白质、22种tRNA、12S和16S rRNA以及两个控制区，大小在17170至17450 bp之间。蓝灰扁尾海蛇的线粒体基因组与扁尾海蛇的线粒体基因组比与半环扁尾海蛇的线粒体基因组更相似。估计这三个扁尾海蛇分支之间的分歧时间为800至1000万年前，并且发现蓝灰扁尾海蛇和扁尾海蛇之间存在密切的系统发育关系。我们的结果有助于我们了解海蛇的进化历史。
