Nieboer P, Hartog A F, Berden J A
Laboratory of Biochemistry, University of Amsterdam, The Netherlands.
Biochim Biophys Acta. 1987 Nov 19;894(2):277-83. doi: 10.1016/0005-2728(87)90197-6.
Mitochondrial F1, inactivated to various extents with 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl), was dissociated with LiCl and reconstituted after removal of the salt. This procedure resulted in a reactivation that corresponded with a reactivation theoretically expected on the basis of the assumption that the reassociation of beta-subunits into native F1 molecules is random and that two out of the three beta-subunits are directly involved in catalysis. Repeated inactivation of such reactivated F1, followed by the same dissociation-association procedure, resulted in similar data. After inactivation of F1 by covalent binding of 2-N-AT(D)P to one catalytic site, no reactivation upon dissociation-reassociation was obtained due to the fact that such modified F1 did not dissociate under the experimental conditions used.
用4-氯-7-硝基苯并-2-恶唑-1,3-二氮杂茂（NBD-Cl）在不同程度上使其失活的线粒体F1，用LiCl解离并在除去盐后进行重组。该程序导致了再活化，这与基于β亚基重新缔合成天然F1分子是随机的以及三个β亚基中有两个直接参与催化的假设理论上预期的再活化相对应。对这种再活化的F1进行重复失活，然后进行相同的解离-缔合程序，得到了相似的数据。在用2-N-AT（D）P共价结合到一个催化位点使F1失活后，由于这种修饰的F1在所用实验条件下不解离，因此解离-重组后未获得再活化。
