Tanaka Sachi, Yamamoto Kana, Hamajima Chisato, Takahashi Fuka, Yamada Kazuki, Furuya Kanon, Uyeno Yutaka
Academic Assembly (Institute of Agriculture), Shinshu University.
Graduate School of Science and Technology, Shinshu University.
Microbes Environ. 2017 Sep 27;32(3):268-274. doi: 10.1264/jsme2.ME17059. Epub 2017 Sep 12.
We aimed to investigate the effects of feeding fermented Brassica rapa L. on ecological and immunological changes in the mouse gut using in vitro cultivation tests and in vivo experiments in normal mice. In the preliminary in vitro study, two B. rapa L. products from different fermentation periods (one d [SF] or six months [LF]) were evaluated along with non-fermented vegetables (NF). Among the components of each product, the insoluble fraction resulted in the most prominent change such as a relative increase in butyrate production during a cultivation inoculated with mouse cecum contents. Based on this result, the boiled water-insoluble fractions of B. rapa L. (SF, LF, and NF samples) were selected as test materials for the subsequent in vivo experiment. Male C57BL/6J mice were divided into four groups and fed either a control diet (CON) or control diet plus one of the insoluble fractions for two weeks. The NF and LF groups had higher relative populations of Faecalibacterium prausnitzii than the CON group. Therefore, colonic butyrate concentrations were higher in the NF and LF groups than in the CON group. The oral administration of B. rapa L. extract induced immune regulatory effects, even when mice were fed NF and SF, but not LF, as assessed by an increase in regulatory T cell numbers. Our results indicate that feeding a purified insoluble fraction from B. rapa L. affects enteric short-chain fatty acid production and immunological responses in the mouse gut in a similar manner, regardless of the fermentation status.
我们旨在通过体外培养试验和正常小鼠体内实验，研究喂食发酵的芜菁对小鼠肠道生态和免疫变化的影响。在初步的体外研究中，对来自不同发酵期（1天[SF]或6个月[LF]）的两种芜菁产品以及未发酵蔬菜（NF）进行了评估。在每种产品的成分中，不溶性部分导致了最显著的变化，例如在接种小鼠盲肠内容物的培养过程中丁酸盐产量相对增加。基于这一结果，选择芜菁的水煮不溶性部分（SF、LF和NF样品）作为后续体内实验的测试材料。将雄性C57BL/6J小鼠分为四组，分别喂食对照饮食（CON）或对照饮食加一种不溶性部分，持续两周。NF组和LF组的普拉梭菌相对丰度高于CON组。因此，NF组和LF组的结肠丁酸盐浓度高于CON组。通过调节性T细胞数量的增加评估，即使小鼠喂食NF和SF而非LF，口服芜菁提取物也能诱导免疫调节作用。我们的结果表明，喂食纯化的芜菁不溶性部分会以类似的方式影响小鼠肠道中的肠道短链脂肪酸产生和免疫反应，而与发酵状态无关。
